Application: detection of binding of biological and / or chemical components of liquid or gaseous mixtures and solutions, which are of mainly biological origin and / or determine parameters of living activity of
biological objects, to substances that bind the said components due to a biological, chemical or
physical interaction; and analysis of mixtures and solutions to determine presence of biological and / or chemical components. Essence: binding substances are arranged on a surface of or inside a sensor layer, which changes its thickness due to the binding being detected; the layer is affected by light of different wavelengths; a
signal due to interference on the sensor layer is registered in the reflected or
transmitted light. In the first variant, the
signal is represented by a spectrum; the sensor layer is more than 10 mum thick and exceeds the maximal recorded
wavelength by at least an
order of magnitude; information about the binding being detected is obtained from analysis of a
spectral shift of interference maximums and minimums. In the second variant, the light passes also a scanned Fabry-Perot interferometer; the recorded
signal is a dependence of intensity of the resulting light upon changes of base of the scanned Fabry-Perot interferometer, in which maximums due to correlation of the spectral characteristics of interaction of the light with the sensor layer and interferometer are observed; information about the binding being detected is obtained from a shift of the said dependence along values of base. In the third variant, other interferometers are used, which are scanned due to a change of the path difference of interfering beams. The required technical result is to make measurement results independent from uncontrollable variations of intensity of the analyzed light in whole as well as in any part of its spectrum, any areas of the sensor layer, and, consequently, to increase accuracy of measurements and reliability of results, sensitivity and resolution with simultaneous reduction of the number of necessary operations, of labor input and cost of both single- and multi-channel variants including real-time registration.