18 results about "Agalactosis" patented technology

This invention discloses a strain of Bifidobacterium animalis subspecies TRT-2A with allergy-relieving effects and its extracellular vesicle applications. The strain TRT-2A is deposited at the China Center for Type Culture Collection (CCTCC) on November 21, 2025, with accession number CCTCC NO:M 20252645. This strain is highly acid-resistant and exhibits good tolerance to artificial gastric and intestinal fluids. It effectively inhibits bacteria, particularly Streptococcus pyogenes, Streptococcus agalactiae, and / or Escherichia coli. Experimental results show that this strain can alleviate hormone-dependent dermatitis and effectively improve dermatitis symptoms such as erythema and desquamation. Furthermore, extracellular vesicles prepared using TRT-2A can effectively improve skin elasticity and reduce wrinkles. Therefore, the Bifidobacterium animalis subspecies TRT-2A of this invention has multiple functions and is worthy of further promotion and application.

The application discloses a kind of fluorescence colorimetric double-mode detection streptococcus agalactiae without aerosol pollution method, comprising the following steps: (1) strain culture and DNA extraction;(2) the combination of UDG and LAMP;(3) UDG-LAMP-CRISPR fluorescence mode detection system;(4) colorimetric mode detection system: read image RGB value in G value, G value significantly increases, and it is positive result.This patent innovatively combines UDG, LAMP, CRISPR and G4, introduces connection probe to realize "fluorescence-colorimetric" single system double-mode detection.The two detection modes of the detection platform can be freely split or combined, and can meet the needs of different detection environments.By combining UDG system and LAMP technology, aerosol pollution possibly carried in amplification process is avoided.Connection probe is introduced, CRISPR fluorescence detection is combined with G4 colorimetric detection, a detection system with "fluorescence-colorimetric" two detection modes is established, which is used for quantitative identification and cross verification of streptococcus agalactiae.

The present application relates to the field of biotechnology, and particularly relates to a KASP molecular marker related to the resistance of tilapia to streptococcus agalactiae disease and an application thereof.Two SNP sites (SNP-1 and SNP-2) significantly associated with the resistance are screened from the intron region of EGFRX2 gene of tilapia, a typing method of the above sites is developed through KASP technology, and the correlation between the SNP-1 genotype and the resistance phenotype is verified.Further, an advantage haplotype individual with the resistance to streptococcus agalactiae disease is screened by combining the two SNP sites, and the survival rate of the individual in the challenge experiment is significantly improved.The detection kit provided by the present application can realize high-throughput and high-precision typing, is suitable for the identification of the resistance genotype of tilapia and the marker-assisted selection, provides an efficient genetic improvement tool for the disease-resistant breeding of tilapia, and is helpful to reduce the loss of breeding diseases and reduce the dependence on antibiotics.

The application discloses a recombinant strain and a preparation method thereof and application of the recombinant strain in preparation of a vaccine for Streptococcus agalactiae of tilapia, and the recombinant strain is successfully constructed by the following steps: designing an ORF4 primer, amplifying an ORF4 gene fragment from a DNA template of Streptococcus agalactiae, editing the ORF4 gene fragment into Escherichia coli by means of a CRISPR / Cas9 gene editing technology, and connecting a green fluorescent protein (GFP). The recombinant strain is subjected to immunoprotection research on the Streptococcus agalactiae of tilapia, and it is shown that the recombinant strain has a good protection effect, and can provide a solid theoretical basis and technical support for application and development of the vaccine for Streptococcus agalactiae of tilapia in the field of aquaculture.

The invention discloses a bifidobacterium animalis subsp. Lactis BT-G9 with a growth promoting function and application of the bifidobacterium animalis subsp. Lactis BT-G9, the bifidobacterium animalis subsp. Lactis BT-G9 is preserved in China Center for Type Culture Collection, the preservation date is November 20, 2025, and the preservation number is CCTCC NO: M 20252626. The brand new bifidobacterium animalis subsp. Lactis BT-G9 provided by the invention has good tolerance to gastric juice and intestinal juice, is suitable for survival in gastrointestinal tracts, can effectively inhibit bacteria, and particularly can effectively inhibit campylobacter kirscheri, streptococcus agalactiae and / or propionibacterium acnes. Experimental results show that the strain can promote animal growth and development and improve the autism, especially when the animal bifidobacterium subsp. Lactis BT-G9 and the lactobacillus acidophilus BT-C15 are combined for use, the animal bifidobacterium subsp. Lactis BT-G9 and the lactobacillus acidophilus BT-C15 have a synergistic effect, and the improvement effect on the autism can be remarkably improved. In addition, the extracellular vesicles prepared from the strain can effectively repair the skin barrier function.

A bacteriophage lytic enzyme and its application. The name of the bacteriophage lytic enzyme is PlyNJ3; the gene fragment encoding the lytic enzyme PlyNJ3 is amplified from streptococcus suis by PCR, and a prokaryotic expression vector plasmid pET32a-PlyNJ3 containing the PlyNJ3 gene is constructed, the recombinant plasmid is obtained by transforming DH5 alpha competent cells, and the protein is massively expressed in an escherichia coli expression system. Then, the lytic enzyme PlyNJ3 is purified by affinity purification medium, the high-efficiency expression of the lytic enzyme PlyNJ3 in vitro is realized, and the lytic effect of the lytic enzyme PlyNJ3 on different serotypes of streptococcus suis, streptococcus agalactiae and other common streptococci is verified in vitro. The bacteriophage lytic enzyme PlyNJ3 prepared by the application can selectively lyse target pathogenic bacteria, and has a good application prospect in the development of new bacteriophage drugs.

The application belongs to the technical field of Chinese herbal medicines, and particularly relates to a Chinese herbal medicine composition for resisting Streptococcus agalactiae, a preparation method and application thereof. Raw materials for preparing the Chinese herbal medicine composition include cortex mali and cumin. The Chinese herbal medicine composition is easy to obtain and simple to prepare, has no chemical residues, is not prone to drug resistance, has significant bacteriostatic activity on Streptococcus agalactiae, and can be applied to the prevention and treatment of Streptococcus agalactiae disease of fish such as tilapia.

The invention relates to immunogenic compositions comprising a capsular polysaccharide (CP) from Streptococcus agalactiae, commonly referred to as group B streptococcus (GBS), conjugated to a carrier protein, and optionally including an aluminum-based adjuvant. The invention further relates to methods for inducing an immune response in subjects against GBS and / or for reducing or preventing invasive GBS disease in subjects using the compositions disclosed herein. The resulting antibodies can be used to treat or prevent GBS infection via passive immunotherapy.

The invention relates to immunogenic compositions comprising a capsular polysaccharide (CP) from Streptococcus agalactiae, commonly referred to as group B Streptococcus (GBS), conjugated to a carrier protein, and optionally including an aluminum-based adjuvant. The invention further relates to methods for inducing an immune response in subjects against GBS and / or for reducing or preventing invasive GBS disease in subjects using the compositions disclosed herein. The resulting antibodies can be used to treat or prevent GBS infection via passive immunotherapy.

The invention discloses lactobacillus acidophilus BT-C15 with inflammation relieving and mucosal barrier repairing functions and application of the lactobacillus acidophilus BT-C15, the lactobacillus acidophilus BT-C15 is preserved in the China Center for Type Culture Collection, the preservation date is November 20, 2025, and the preservation number is CCTCC NO: M 20252625. The invention provides a brand new lactobacillus acidophilus BT-C15 which mainly has the effects of relieving inflammation and whitening the skin. The lactobacillus acidophilus BT-C15 has good tolerance to gastric juice and intestinal juice, is high in cell adhesion capacity, is suitable for survival in gastrointestinal tracts, can effectively inhibit bacteria, and particularly can effectively inhibit campylobacter Korotkoff, streptococcus agalactiae and / or propionibacterium acnes. Experimental results show that the strain can improve colitis, fade spots, whiten skin and improve autism, especially when the lactobacillus acidophilus BT-C15 and the bifidobacterium animalis subsp. Lactis BT-G9 are used in a combined mode, the lactobacillus acidophilus BT-C15 and the bifidobacterium animalis subsp. Lactis BT-G9 have a synergistic effect, and the improvement effect on the autism can be remarkably improved.

A method for rapidly identifying highly virulent strains of Streptococcus agalactiae, comprising: using a laser confocal Raman spectrometer to collect single-cell Raman spectra of clinical strains, and establishing a database; classifying data of III / ST17-type highly virulent GBS strains into a first group, named as GBS-HIGH, data of non-III / ST17-type GBS strains into a second group, named as GBS-OTHERS, and data of Escherichia coli into a third group, named as ECO; randomly dividing the three groups of data into a training set, a validation set and a test set in a ratio of 7:1:2; and on the basis of a ResNet18 residual neural network architecture, changing 2D convolution in a network to one-dimensional (1D) convolution suitable for a 1D Raman spectrum, determining optimal parameters by means of simulation experiments, training the weight of a model in the training set, and then selecting optimal hyperparameters by means of a result of the model on the verification set, so as to determine a trained model.

The application discloses a kind of nanobody targeting streptococcus agalactiae SIP protein and preparation and application thereof, it is related to biotechnology field, and the nanobody that the present application is screened by nanobody phage library construction, phage screening technology, and is efficiently and specifically combined with SIP protein Nanobody, the nanobody of streptococcus agalactiae SIP protein includes VHH fragment, with the amino acid sequence shown in SEQ ID NO:1.The nanobody can be combined with streptococcus agalactiae SIP protein high specificity, and the antibody can be targeted to SIP protein by the antibody, and the application discloses a kind of nanobody targeting streptococcus agalactiae SIP protein can be applied to fish disease diagnosis and treatment.

The application provides a tilapia antibacterial peptide OnLEAP-2 and an application thereof, and an antibacterial peptide named OnLEAP-2 is cloned from tilapia, and a large amount of the antibacterial peptide is obtained through prokaryotic expression. The antibacterial peptide has a good effect of resisting Streptococcus agalactiae, so that the survival rate of the tilapia is significantly improved, and pathogens are efficiently removed. The antibacterial peptide is suitable for the prevention and treatment of bacterial diseases of economic fishes such as tilapia.

The present application belongs to the technical field of genetic engineering, and particularly relates to a Streptococcus agalactiae Mu-20 and a construction method and application thereof. The present application constructs a fish-derived Streptococcus agalactiae strain with a capsule polysaccharide deficiency, no hyaluronidase activity, no hemolysis and no trehalose fermentation, and the strain number is Mu-20. The strain was preserved in the Guangdong Microbial Culture Collection Center on June 13, 2022, and the preservation number is GDMCC No: 62540. The strain does not carry a chloramphenicol resistance marker gene, has high biological safety, has no risk of virulence returning to strength, has high immunogenicity, can protect immunized fish against the invasion of Streptococcus agalactiae, and is easy to cultivate, simple to use and low in production cost. The strain involved in the present application can be used to prepare a live attenuated vaccine for fish streptococcosis, and has extremely high commercial application value.

The invention discloses lactobacillus crispatus GG77 capable of repairing mucosal lesion and preventing pathogenic bacterium infection and application of the lactobacillus crispatus GG77, the lactobacillus crispatus GG77 is preserved in the China Center for Type Culture Collection, the preservation date is November 21, 2025, and the preservation number is CCTCC NO: M 20252646. Compared with the prior art, the invention provides a brand-new lactobacillus crispatus GG77 which mainly has the effects of repairing mucosal lesion and preventing pathogenic bacterium infection. The lactobacillus crispatus GG77 has high acid resistance, can effectively inhibit bacteria, and particularly can effectively inhibit campylobacter Korotkoff, streptococcus agalactiae and / or enterobacter cloacae. Experimental results show that the lactobacillus crispatus GG77 can repair mucosal lesion, prevent vaginitis recurrence, recover genital tract flora and promote calcium ion transport and absorption. Particularly, when the lactobacillus crispatus GG77 and the Witzia clostridium CN365 are combined for use, the lactobacillus crispatus GG77 and the Witzia clostridium CN365 have a synergistic effect, and the effect of transferring and absorbing calcium ions can be remarkably improved.

The invention discloses a freeze-dried reagent ball special for streptococcus agalactiae CRISPR detection aiming at a tilapia mossambica tissue matrix and application of the freeze-dried reagent ball, and belongs to the field of molecular diagnosis technology and on-site rapid detection of aquatic diseases. The freeze-drying reagent ball is a three-layer spherical freeze-drying body comprising an inner core layer, an isolating layer and a shell layer, the inner core layer contains an RAA amplification component aiming at a streptococcus agalactiae cfb gene, the shell layer contains a CRISPR-Cas12a detection component and a special anti-inhibition buffer system, and the isolating layer is a solid freeze-drying material layer of which the dissolution rate is slower than that of the shell layer. Through the dissolution time sequence control of the three-layer structure, the automatic sequence that an outer shell layer firstly dissolves and neutralizes an inhibitor and presets a detection system and an inner core layer then dissolves and starts amplification is realized, and multi-step reaction is integrated into one-step closed tube operation. During detection, only tilapia tissue crude extract needs to be added, and a result is interpreted through fluorescence after constant-temperature incubation. The method effectively overcomes the problems of aquatic product tissue matrix inhibition, unstable operation of an existing single-tube method and on-site pollution risk, and is high in sensitivity, extremely simple to operate and stable at normal temperature.

This invention discloses a triplet real-time PCR detection method for Streptococcus agalactiae, Streptococcus dolphinus, and Lactococcus gasseri. It utilizes three primer sets and probes: one for Streptococcus agalactiae, one for Streptococcus dolphinus, and one for Lactococcus gasseri. By optimizing the degradation temperature, primer dosage, and probe dosage, this invention effectively reduces the mutual interference of the three target pathogens, improving the accuracy of the detection results. This detection method has significant application value in fish farming. The reagent kit of this invention has low preparation cost, a simple preparation process, and is easily scalable for industrial production, thus possessing broad market prospects.