The invention discloses a high-speed transmission method based on a PCIE interface, a storage medium and a terminal, and the method comprises the steps: judging whether a function library exists in anFPGA prototype verification development board or not, and initializing the function library after judging that the function library exists; calling a device to search and open an interface, enumerating all PCIe devices in the current terminal, and opening an equipment CAPI; carrying out register reading and writing on the designated address, then, judging whether data comparison is correct or notaccording to a reading and writing result, if so, carrying out DMA transmission on the designated address, carrying out comparison and processing on the data, and then, turning off the equipment CAPI; and if the data comparison result is judged to be wrong, directly closing the equipment CAPI and stopping transmission, so that a large amount of data can be transmitted between a host and a prototype at a high speed, the real-time test is approached, the user design can be executed at a hardware speed, the design verification is accelerated, the test coverage of FPGA prototype verification is increased, and the problems proposed in the background technology can be effectively solved.