99 results about "Hypoplastic philtrum" patented technology

The present invention discloses pharmaceutical compositions for the treatment of skeletal dysplasias, comprising as an active ingredient at least one natriuretic peptide. Unexpectedly, it has been shown that the natriuretic factors may be effective for bone elongation in situations of abnormal bone growth especially for achondroplasia. The effects of the natriuretic peptide may be further enhanced by prolonging its residence time or action at the target site.

The present invention comprises, without limitation, systems, methods, and compositions for the detection, identification, and quantification, down to the single copy level, of human papillomavirus (HPV) in biological samples, including but not limited to, mammalian body fluids and cervix scrapings, for purposes of detection, treatment and / or management of cancer and dysplasia.

Transgenic chloroplast technology could provide a viable solution to the production of Insulin-like Growth Factor I (IGF-I), Human Serum Albumin (HSA), or interferons (IFN) because of hyper-expression capabilities, ability to fold and process eukaryotic proteins with disulfide bridges (thereby eliminating the need for expensive post-purification processing). Tobacco is an ideal choice because of its large biomass, ease of scale-up (million seeds per plant), genetic manipulation and impending need to explore alternate uses for this hazardous crop. Therefore, all three human proteins will be expressed as follows: a) Develop recombinant DNA vectors for enhanced expression via tobacco chloroplast genomes b) generate transgenic plants c) characterize transgenic expression of proteins or fusion proteins using molecular and biochemical methods d) large scale purification of therapeutic proteins from transgenic tobacco and comparison of current purification / processing methods in E. coli or yeast e) Characterization and comparison of therapeutic proteins (yield, purity, functionality) produced in yeast or E. coli with transgenic tobacco f) animal testing and pre-clinical trials for effectiveness of the therapeutic proteins. Mass production of affordable vaccines can be achieved by genetically engineering plants to produce recombinant proteins that are candidate vaccine antigens. The B subunits of Enteroxigenic E. coli (LTB) and cholera toxin of Vibrio cholerae (CTB) are examples of such antigens. When the native LTB gene was expressed via the tobacco nuclear genome, LTB accumulated at levels less than 0.01% of the total soluble leaf protein. Production of effective levels of LTB in plants, required extensive codon modification. Amplification of an unmodified CTB coding sequence in chloroplasts, up to 10,000 copies per cell, resulted in the accumulation of up to 4.1% of total soluble tobacco leaf protein as oligomers (about 410 fold higher expression levels than that of the unmodified LTB gene). PCR and Southern blot analyses confirmed stable integration of the CTB gene into the chloroplast genome. Western blot analysis showed that chloroplast synthesized CTB assembled into oligomers and was antigenically identical to purified native CTB. Also, GM1,-ganglioside binding assays confirmed that chloroplast synthesized CTB binds to the intestinal membrane receptor of cholera toxin, indicating correct folding and disulfide bond formation within the chloroplast. In contrast to stunted nuclear transgenic plants, chloroplast transgenic plants were morphologically indistinguishable from untransformed plants, when CTB was constitutively expressed. The introduced gene was stably inherited in the subsequent generation as confirmed by PCR and Southern blot analyses. Incrased production of an efficient transmucosal carrier molecule and delivery system, like CTB, in transgenic chloroplasts makes plant based oral vaccines and fusion proteins with CTB needing oral administration a much more practical approach.

The present invention relates to a method for the treatment of skeletal dysplasia by administering to a patient a composition comprising a therapeutically effective amount of at least one C-type natriuretic peptide (CNP).

The invention relates to newly discovered nucleic acid molecules and proteins associated with cervical cancer including pre-malignant conditions such as dysplasia. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human cervical cancers are provided.

This invention provides methods and kits for improved diagnosis of medically relevant conditions by solution based biochemical testing procedures performed in solutions of test samples. The invention provides a method to substitute the cell based morphological information contained within the cytological and / or histological data of the test sample by molecular information obtainable from the solution, wherein the original test sample is dissolved and thus enables for accurate and reproducible assessment of medically relevant diagnosis from dissolved test samples. The method according to the invention comprises the steps of determining the levels of one or more disease markers associated with the condition to be diagnosed, determining the level of one or more normalization markers suitable to substitute the information related to morphological aspects of the sample, comparing and / or combining the data of the disease and normalization markers, and assessing diagnosis of a medically relevant condition.

New compositions are disclosed that comprise the phytochemical Diindolylmethane (DIM), as well as its precursor, Indole-3-carbinol (I3C), and cogener, 2-(Indol-3-ylmethyl)-3,3′diindolylmethane (LTr-1), acceptable carriers and / or excipients. These compositions are administered to prevent or reduce symptoms associated with mastalgia, endometriosis and HPV-related conditions including cervical dysplasia.

The invention discloses a breeding method of breeding pigs, which comprises the following steps of: primary selection: excluding pigs which have the genetic defects, do not comfort to the appearance features and are poor in growth and development at the condition of 30 kg; secondary selection: carrying out elimination treatment or other treatments on individuals which are poor in development and are not qualified under the condition of 60 kg; tertiary selection: calculating the selection index of each pig according to the backfat thickness, the growth speed and other data, carrying out site selection according to high and low indexes and in combination with the blood relationship and the body type appearance and selecting pigs with high indexes and good body type appearance into a core group; starting to mate after the weights and the month ages of gilts subjected to the tertiary selection reach the standard; and carrying out semen examination and limb and hoof compactness evaluation on boars selected by final detection before mating and eliminating the boars which are not qualified in the continuous tertiary semen examination. The breeding pigs bred by adopting the method disclosed by the invention have the advantages of high pig fecundity and quick weight-increasing speed and are very suitable for being raised and reproduced by various farming village farmers.

A biphasic lipid vesicle composition for treating cervical displasia by intravaginal delivery. The composition includes a suspension of lipid-bilayer vesicles having entrapped therein, an oil-in-water emulsion, human interferon alpha-2b and L-methionine, the composition having an interferon alpha-2b specific activity of between about 1-10 MIU (million international units) per gram composition, and between 0.01 to 0.5 weight percent L-methionine. In the treatment method, the composition is administered at a dose of between about 1-20 MIU interferon alpha-2b, and this dose is administered at least 3 days / week, for a period of at least 4 weeks.

The present invention, in one aspect, relates to a method of and apparatus for examining a target for tumors or lesions using what is referred to as ''Early Increase in microvascular Blood Supply'' (EIBS) that exists in tissues that are close to, but are not themselves, the abnormal tissue and in tissues that precede the development of such lesions or tumors. While the abnormal tissue can be a lesion or tumor, the abnofmal tissue can also be tissue that precedes formation of a lesion or tumor, such as a precancerous adenoma, aberrant crypt foci, tissues that precede the development of dysplastic lesions that themselves do not yet exhibit dysplastic phenotype, and tissues in the vicinity of these lesions or pre-dysplastic tissues.

The invention discloses an ion-type rare-earth seepage control in-situ mining method for solving the problems of immersion liquid leakage, landslide and ecological damage in an existing in-situ leaching process of an ion adsorption type rare earth mine. The ion-type rare-earth seepage control in-situ mining method is characterized in that a comprehensive geophysical prospecting method is introduced into the existing in-situ leaching process for detecting fault, fracture and crushing development conditions of a bed rock bottom plate; a grouting technology is introduced into the existing in-situleaching process to construct an impervious layer on the bed rock bottom plate with poor development, so that the loss of rare-earth resources, caused by the fact that immersion liquid enters underground water through the bed rock bottom plate, is prevented; and an injection-extraction self-control system composed of an infiltration line automatic monitoring sensor, an automatic liquid injectiondevice and vacuum extraction equipment is introduced into the existing in-situ leaching process, so that the injection-extraction of the immersion liquid is accurately controlled, and the constructionof a digital mine is assisted. The method is particularly suitable for the ion adsorption type rare earth mines with poor bottom plate development and poor permeability.

A biphasic lipid vesicle composition for treating cervical displasia by intravaginal delivery. The composition includes a suspension of lipid-bilayer vesicles having entrapped therein, an oil-in-water emulsion, human interferon alpha-2b and L-methionine, the composition having an interferon alpha-2b specific activity of between about 1-10 MIU (million international units) per gram composition, and between 0.01 to 0.5 weight percent L-methionine. In the treatment method, the composition is administered at a dose of between about 1-20 MIU interferon alpha-2b, and this dose is administered at least 3 days / week, for a period of at least 4 weeks.

Transgenic chloroplast technology could provide a viable solution to the production of Insulin-like Growth Factor I (IGF-I), Human Serum Albumin (HSA), or interferons (IFN) because of hyper-expression capabilities, ability to fold and process eukaryotic proteins with disulfide bridges (thereby eliminating the need for expensive post-purification processing). Tobacco is an ideal choice because of its large biomass, ease of scale-up (million seeds per plant), genetic manipulation and impending need to explore alternate uses for this hazardous crop. Therefore, all three human proteins will be expressed as follows: a) Develop recombinant DNA vectors for enhanced expression via tobacco chloroplast genomes b) generate transgenic plants c) characterize transgenic expression of proteins or fusion proteins using molecular and biochemical methods d) large scale purification of therapeutic proteins from transgenic tobacco and comparison of current purification / processing methods in E. coli or yeast e) Characterization and comparison of therapeutic proteins (yield, purity, functionality) produced in yeast or E. coli with transgenic tobacco f) animal testing and pre-clinical trials for effectiveness of the therapeutic proteins. Mass production of affordable vaccines can be achieved by genetically engineering plants to produce recombinant proteins that are candidate vaccine antigens. The B subunits of Enteroxigenic E. coli (LTB) and cholera toxin of Vibrio cholerae (CTB) are examples of such antigens. When the native LTB gene was expressed via the tobacco nuclear genome, LTB accumulated at levels less than 0.01% of the total soluble leaf protein. Production of effective levels of LTB in plants, required extensive codon modification. Amplification of an unmodified CTB coding sequence in chloroplasts, up to 10,000 copies per cell, resulted in the accumulation of up to 4.1% of total soluble tobacco leaf protein as oligomers (about 410 fold higher expression levels than that of the unmodified LTB gene). PCR and Southern blot analyses confirmed stable integration of the CTB gene into the chloroplast genome. Western blot analysis showed that chloroplast synthesized CTB assembled into oligomers and was antigenically identical to purified native CTB. Also, GM1-ganglioside binding assays confirmed that chloroplast synthesized CTB binds to the intestinal membrane receptor of cholera toxin, indicating correct folding and disulfide bond formation within the chloroplast. In contrast to stunted nuclear transgenic plants, chloroplast transgenic plants were morphologically indistinguishable from untransformed plants, when CTB was constitutively expressed. The introduced gene was stably inherited in the subsequent generation as confirmed by PCR and Southern blot analyses. Incrased production of an efficient transmucosal carrier molecule and delivery system, like CTB, in transgenic chloroplasts makes plant based oral vaccines and fusion proteins with CTB needing oral administration a much more practical approach.

The invention relates to a traditional Chinese medicine composition for treating uterine hypoplasia, a preparation method thereof and application of the composition in preparation of a medicine used for treating uterine hypoplasia. The composition comprises the following bulk drugs: human placenta, dodder seed, epimedium herb, barbary wolfberry fruit, morinda root, degelatined deer-horn, fluorite, desertliving cistanche, tortoise carapace and plastron, asiatic cornelian cherry fruit, tuber onion seed, Chinese angelica, cibot rhizome, Indian bread, common yam rhizome, debark peony root, ginseng and largehead atractylodes rhizome. The traditional Chinese medicine composition can be prepared by using a conventional traditional Chinese medicine preparation method to obtain a clinically applicable preparation; preferably, the preparation is an oral preparation or an externally applied preparation; and more preferably, the oral preparation is a decoction, and the externally applied preparation is an externally applied powder. The invention has the following characteristics: the traditional Chinese medicine composition has a remarkable curative effect no toxic and side effects, and the preparation method is simple.

The invention relates to an agent for treating cervical dysplasias, comprising a recombinant, genetically modified E1-deleted adenovirus replication defective in non-HPV infected cells, which is suitable for local external application in the region of the portio and the cervix uteri.

The present invention is directed to a set of tableware for use by persons with neuro-cognitive impairment, compromised or under developed motor skills, or any persons with difficulties eating foods and imbibing drinks. As described herein, the tableware set includes at least one container and at least one spoon. The container has a smooth sidewall portion, a vertically slanted basin portion to define an asymmetrical cavity enclosed by the container. A flat base is provided on the underside of the container. A spoon is provided to match the curvature of the container along or more axes. To assist disabled persons with using the spoon, the side edges of the spoon matches the vertical curvature of the sidewall container, and the tip of the spoon matches the horizontal curvature. The handle of the spoon is designed along an arc to match the alignment of a closed hand when holding a spoon.

The invention relates to a high-yield hybrid seed production method for sweet pepper, and relates to the technical field of hybrid seed production. A technical solution comprises the steps of firstly finishing maternal plants; removing all blossomed flowers from the plants; pruning to removal all branches below door pepper and branches that are weak and hypogenetic; selecting flower buds to be blossomed in the next day from a second layer flower to a forth layer flower; emasculating after 5 o'clock in the afternoon; harvesting mature but non-cracked anther in the morning a day before fertilization or at 7-10 o'clock in the morning; drying the harvested anther in a drier for use in fertilization; dipping pollen with a writing brush or eraser head at 7-10 o'clock in the morning; coating emasculated stigma with the pollen; and harvesting in 50-70 days after the fertilization when the fruits are fully mature. The method increases purity, yield and seed plumpness for the seed production of the sweet pepper.

Oral compositions comprising an association of one or of a mixture of phytoestrogens, selective for the estrogen receptor-β, with dietary fibres are described. The compositions thereof can be profitably used for the prophylactic and therapeutic treatment in mammals, including humans, of health conditions characterised by a high risk of onset and recurrence of intestinal adenomas (adenopolyposis coli), and of adenoma's progression to colorectal carcinoma. In presence of pre-cancerous lesions in the colon, characterised by mutations of the APC tumor suppressor and defined as polyps or adenomas, the oral compositions disclosed can be in fact profitably used to reduce the number and volume of polyps, and to prevent their progression to neoplastic transformation, reducing their degree of dysplasia.

Compositions and methods for modulating tumor proliferation in an individual are provided. The methods employ nucleolin-binding agents, such as aptamers. The aptamers of the present invention can be used to modulate the proliferation of malignant, dysplastic; hyperproliferative, and / or metastatic cells through interference with molecular interactions and functions of nucleolin in the tumor cell.

An method for selective breeding of a fast-growing strain of lined seahorse, including: (1) selecting high-quality lined seahorse parents as a base population for selective breeding; (2) performing prenatal intensified breeding; (3) performing pairing and mating, and rearing the juveniles thereof; (4) performing selective breeding in three junctures with different selection proportions; (5) pairing the saved adult lined seahorses, wherein those with low vitality, having injuries on the body surface, or having a dysplastic brood pouch or gonad are culled, and a selection proportion of 10% for every generation is maintained thereafter; (6) repeating steps (2) through (5) for at least four times to obtain a high-quality strain of lined seahorse with improved weight and body length and stable growth. The method can be used for selective breeding of a new strain with high quality and high yield.

The invention discloses a high-yield hybrid seed production method for tomatoes, and relates to the technical field of hybrid seed production. According to the technical scheme adopted by the invention, the high-yield hybrid seed production method comprises the following steps: firstly, trimming maternal plants to remove all flowers in blossom on the plant, and simultaneously pruning to remove all branches and internal branches which are thin and weak, and hypoplastic under the first fruit; starting to select flower buds to be in blossom next day from the second layer to the fourth layer of flowers, and carrying out castration after 5 o'clock in that afternoon; in the morning a day before pollination or at 7 o'clock to 10 o'clock in that morning, collecting mature and non-crack anthers, and drying the collected mature and non-crack anthers in a dryer for later pollination use; staining a writing brush or an eraser head with pollen preferably from 7 o'clock to 10 o'clock in the morning in general, and lightly coating the pollen on flower stigmas subjected to castration; and harvesting the fully-matured fruits about 50-70 days after pollinating. According to the high-yield hybrid seed production method for tomatoes, the purity and the yield for tomato seed production and the plumpness of the seeds are improved.

The invention discloses a rearing method for correcting dysplasia of laying hens at a growing period, which deeply studies how to correct the dysplasia of body types of the laying hens caused by rearing deficiency during brooding through compensation feed at the growing period of the laying hens aged 7 to 18 weeks, and further allows the actual body type development index of the laying hens at the end of the growing period to reach the target body type development index as much as possible, and lays the foundation for the laying hens to achieve high yield. Compared with the conventional rearing method, the method has the advantages that: the rates of reaching to the standards of the body weight and the shank length of pullets aged 19 weeks respectively increase by 11.3% and 8.7%, and the uniformity of hen populations increases by 9.2%; the egg production increases by 8.7%, and the mortality rate reduces by 5.7% at the laying period; and the hen raising efficiency increases by 17.6%.

A high-yield hybrid tomato seed production method relates to the technical field of hybrid seed production. The technical scheme of the method is as follows: firstly arranging maternal plants, removing all blooming flowers on the maternal plants, arranging branches at the same time and removing all branches and twigs thin and weak in interior and poor in growth below gate peppers; selecting the second to fourth layers of buds, which are about to bloom in the next day, and performing emasculation after 5 PM in the same day; collecting and drying ripe and uncracked anthers in a drier for standby of pollination in the morning of the day before pollination or during a time period ranging from 7 AM to 10 AM in the same day, generally, during an appropriate time period ranging from 7 AM to 10 AM, using a writing brush or rubber head to dip pollen and gently coating the pollen on the stigmas subjected to emasculation; picking fully ripe fruits after 50-70 days of pollination. The high-yield hybrid tomato seed production method improves the purity and yield of tomato seed production and the degree of fullness of seeds.

Oral compositions comprising an association of one or of a mixture of phytoestrogens, selective for the estrogen receptor-β, with dietary fibres are described. The compositions thereof can be profitably used for the prophylactic and therapeutic treatment in mammals, including humans, of health conditions characterised by a high risk of onset and recurrence of intestinal adenomas (adenopolyposis coli), and of adenoma's progression to colorectal carcinoma. In presence of pre-cancerous lesions in the colon, characterised by mutations of the APC tumor suppressor and defined as polyps or adenomas, the oral compositions disclosed can be in fact profitably used to reduce the number and volume of polyps, and to prevent their progression to neoplastic transformation, reducing their degree of dysplasia.

A high-yield hybrid cotton seed production method relates to the technical field of hybrid seed production. The technical scheme of the method is as follows: firstly arranging maternal plants, removing all blooming flowers on the maternal plants, arranging branches at the same time and removing all branches and twigs thin and weak in interior and poor in growth below gate peppers; selecting the second to fourth layers of buds, which are about to bloom in the next day, and performing emasculation after 5 PM in the same day; collecting and drying ripe and uncracked anthers in a drier for standby of pollination in the morning of the day before pollination or during a time period ranging from 7 AM to 10 AM in the same day; generally, during an appropriate time period ranging from 7 AM to 10 AM, using a writing brush or rubber head to dip pollen and gently coating the pollen on the stigmas subjected to emasculation; picking fully ripe fruits after 50-70 days of pollination. The high-yield hybrid cotton seed production method improves the purity and yield of cotton seed production and the degree of fullness of seeds.

The invention relates to the technical field of hybrid seed production, in particular to a high-yield hybrid seed production method of cotton. According to the technical scheme, the method includes: sorting female plants, removing full blossoms from the female plants, pruning branches, and removing all branches and inner weak shoots poorly developed below each first fruit; selecting buds, about to blossom the next day, from the second to fourth layers of blossoms on each plant, and performing emasculation after 5 P.M. on the very day; at 7 to 10 in the morning of the previous day or in the morning of the very day, collecting mature non-cracking anthers, and drying them in a dryer for pollination; generally at 7 to 10 in the morning, slightly smearing pollen to emasculated stigmas with a writing brush or a rubber head; harvesting fruits fully mature about 50 to 70 days after pollination. The method has the advantages that produced cotton seeds are higher in purity, yield and fullness.

The disclosure features a human milk cream composition, standardized high fat human milk formulations as well as methods of making and using such compositions. In particular, the disclosure features a method of using a human milk cream composition and / or standardized high fat human milk formulations to treat infants with bronchopulmonary dysplasia (BPD) or at risk of developing BPD.

The invention discloses a planting method capable of increasing sugar content of oranges. The planting method comprises the following steps that (1) a tip fertilizer is applied three times in late February, early March and mid-to-late May every year; (2) flower thinning and fruit thinning are conducted in June and July every year, wherein after stable growth of fruit, deformed fruit, small fruit and stunted fruit are timely removed, and the fruit of normal growth is reserved; (3) in the blooming period of oranges in April and May and the fruit ripening period from October to December, a sugaradding fertilizer is applied every ten days, wherein the sugar adding fertilizer is prepared from, by weight, 200-300 parts of momordica grosvenori water extract solution, 50-100 parts of molasses, 30-80 parts of malt sugar, 50-80 parts of potassium sulphate, 200-300 parts of potassium humate and 1000 parts of water. The planting method can effectively increase the sugar content of oranges, and the commodity value of oranges is improved.