The invention belongs to the field of oil exploration, and particularly relates to a quantitative prediction method for different-scale fractures of a low-permeability reservoir under constraint of a rock mechanics layer, which comprises the following steps: establishing a single-well fracture development mode, establishing a well-connected fracture development mode, dividing fracture scales, performing a rock mechanics experiment, performing mechanical parameter logging interpretation and dynamic-static correction, dividing various levels of rock mechanical layer structures, establishing a three-dimensional geomechanical model, determining a key fracture forming period, performing three-dimensional ancient stress field simulation, deducing and establishing a soft and hard interbed rock multi-stage fracture criterion and a fracture parameter mechanical characterization model, and performing quantitative prediction on fractures of different scales of a low-permeability reservoir, and finally verifying the reliability of a crack quantitative prediction result through well point actual measurement data. According to the method, the multi-scale fracture parameters of the sand-mudstone interbed type low-permeability reservoir can be accurately obtained and quantitatively represented, the method is suitable for fracture quantitative prediction work of any stratified structure reservoir, and the risk and cost of exploration and development are reduced.