The invention discloses a multi-AUV three-dimensional collaborative route planning method. The method mainly comprises the steps of three-dimensional environment abstract modeling, level set functioninitialization, level set function evolution, optimal route point selection, collaborative route scheme design, route conflict judgment and re-planning and planning result output. According to the invention, the level set algorithm is improved; a plurality of airways can be simultaneously planned in a navigation space, thereby improving planning efficiency of algorithms, all AUVs are required to reach the end point at the same time during collaborative scheme design; each AUV needs to start one by one according to delay time, in addition, under the condition of considering rapidity and concealment, a collaborative planning scheme with the concealment and navigation time fused optimally is designed, the influence of ocean current and sound velocity factors is considered at the same time, conflict judgment and re-planning links are added, and a collaborative route is safer; compared with two-dimensional air route planning, the implementation of the three-dimensional air route planning ismore practical, and the actual navigation requirement can be better met.