The present disclosure provides a method for preparing a metal nanostructure through self-priming nanoimprinting. The method comprises the steps that polydimethylsiloxane (PDMS) is used for rollover on the surface of a hard-matrix nanoimprinting template, and a PDMS soft template is obtained; the surface of a substrate is spin-coated with a lower layer of glue, heating and curing are carried out,and the surface of the lower layer of glue is spin-coated with an upper layer of glue; the PDMS soft template is overlaid on the surface of the upper layer of glue, the upper layer of glue is heated and cured, the PDMS soft template is removed, and a grating structure is obtained; if being negative glue, the upper layer of glue is baked after being exposed, and if being positive glue, the upper layer of glue is not exposed; the upper layer of glue and the lower layer of glue on the substrate form a downfall structure by development; and metal is evaporated, the lower layer of glue, the upper layer of glue and metal located on the upper layer of glue are stripped through a stripping process, and the metal nanostructure is formed.