The invention discloses a symmetrical decoupling and single degree of freedom flexible operation mechanism. The symmetrical decoupling and single degree of freedom flexible operation mechanism is characterized in that the flexible operation mechanism comprises the following components connected and assembled according to design requirements: a piezoelectric ceramics driver, a spherical connector, a driving end flexible hinge, a rigid connecting block, four groups of flexible hinge combinations, two flexible chains, a rigid moving platform, a rigid support frame and a base. The mechanism adopting the flexible connecting structure, the single degree of freedom flexible hinges and the piezoelectric ceramics driver driving technology has one actuation terminal, can be applied to micro/nano operation mechanical systems, and has the advantages of being high in precision, low in inertia, compact in structure, free of error accumulation, free of mechanical friction, free of clearance, convenient to process, convenient to manufacture, convenient to industrially implement and the like.