The invention relates to refrigerated dough steamed bread and a production method thereof, which belong to the technical filed of food processing. The method comprises the following steps: firstly, placing flour, yanshan yeast, aluminum-free baking powder, white granulated sugar, shortening oil, salt, transglutaminase, lipase, xylanase, glucose oxidase, trehalose, ascorbic acid, lecithin, sucrose ester, glycerin, locust bean gum, artemisia, carboxymethyl cellulose, guar gum, wheat gluten, wheat starch or cornstarch or potato starch or rice starch into a stirring jar; slowly and uniformly stirring and mixing the mixed materials; then, adding water into the mixed materials for fast stirring to form a dough; then, carrying out dough pressing, forming and quick freezing; next, storing the dough in frozen pack storage for use; taking out the dough when being required; carrying out fermentation; then, steaming the dough; and obtaining fresh steamed bread products. The invention overcomes the problem of flavor loss caused by repeated boiling. When the advantages of uniform color, smooth surface, fluffy mouth feeling, elasticity and the like of the steamed bread (steamed stuffed bun) products are maintained, the product quality standardization, the safety and the convenience can be improved.