31 results about "Heteroantigens" patented technology

The invention relates to a joint detection device and a preparation method of helicobacter pylori urease antibodies IgM and IgG. The helicobacter pylori urease antibodies are prepared from a nitrocellulose membrane, a glass fiber adsorbing a colloidal gold labeled helicobacter pylori urease antigen and a mouse IgG, a sample pad, absorbent paper and other auxiliary materials, wherein the materials are adhered together, and the nitrocellulose membrane contains a purified high-specificity mouse-anti-human IgM and IgG antibodies, and a goat-anti-mouse antibody in a solid phase manner. The joint detection device has the advantages that the structure is simple, the conception is novel, the nitrocellulose membrane are coated with the anti-human IgM and IgG antibodies, so that the specificity is strong, and the helicobacter pylori urease antibodies IgM and IgG in a specimen are simultaneously detected without increasing the production operation complexity. Proper gold spray buffer and sample pad treating fluid are matched to effectively improve the reaction sensitivity on the basis of guaranteeing complete release of immunocolloidal gold, and under the same threshold, the use amount of the immunocolloidal gold can be reduced to save the cost. The detection device is high in sensitivity, strong in specificity, simple, convenient and strong in practicability and can realize the time-saving aim during operation.

The present invention concerns a method for prophylactic and / or therapeutic vaccination and / or treatment and / or diagnosis of HIV / AIDS, other infectious diseases, inflammatory and angiogenic diseases and tumours which utilizes a biologically active HIV-1 Tat protein, fragments or derivates thereof, as a module with one or more of the following features: antigen, adjuvant and targeting-delivery system to specific antigen-presenting cells including dendritic cells, endothelial cells and macrophages. In particular, it is claimed that Tat can be used only in its biologically active form as an antigen, alone or combined with one or more other antigens, to prime or to boost protective immune responses against itself as well as other antigens and / or to deliver these antigen(s) as well as active compounds to dendritic cells, endothelial cells and macrophages, due to its capability of targeting these APC and of activating their maturation and functions and of increasing Th-1 type immune responses as an adjuvant. Therefore, due to these characteristics and to the distribution of these cells in the body (during physiological and pathological disorders), biologically active Tat, fragments or derivates thereof containing the RGD region, can be used for preventive, therapeutic and / or diagnostic purposes for HIV / AIDS, other infectious diseases, inflammatory and angiogenic diseases and tumors.

The invention discloses a colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY). The test strip consists of a sample pad, a colloidal gold pad, an NC film, a piece of water absorption filter paper and a back lining, wherein the colloidal gold pad is enveloped with a TMV specific antibody and a PVY specific antibody which are marked by colloidal gold, and the TMV specific antibody and the PVY specific antibody are respectively generated by secretion by hybridoma cell lines, i.e., BALB / c-15-50 and BALB / c-15-8; the NC film is provided with a detection line and a control line; the detection line is enveloped with specific antigens of the TMV and the PVY; the control line is enveloped with a second antibody marked by the colloidal gold. The rapid detection colloidal gold test strip is especially suitable for the detection on the TMV and the PVY in Guangdong tobacco-growing areas, and is rapid, sensitive and accurate in dection, low in cost and simple and convenient in operation; the test strip can be used to diagnose the two viruses at the same time by one-time sampling, thus being very suitable for field primary screening of a great batch of samples; therefore, the colloidal gold test strip has a good application value in actual production and is good in popularization and application prospects.

The invention provides a detection kit for a prostate specific antigen, a detection method and an application thereof, which relate to the technical field of antigen detection. The kit and the detection method of the invention utilize a secondary antibody to modify DNA, form a Y-shaped antigen-antibody-DNA hybridization chain by means of proximity ligation with the antigen, and converts the antigen input into DNA signal output by using the antigen-antibody-DNA hybridization chain as a signal converter; meanwhile, a chain substitution reaction is combined with a hybridization chain type reaction, and two-stage amplification of the signal is achieved; and every two adjacent two-end sequences (hairpin DNA H3 and hairpin DNA H4) on long-chain DNA are assembled to form a magnesium ion DNA enzyme, the magnesium ion DNA enzyme cuts molecular beacons in a solution in the presence of magnesium ions, the cut-off molecular beacons are released and fluoresce, new molecular beacons are combined with the magnesium DNA enzyme and cut off by the same, and a fluorescent signal is cyclically amplified to realize the third round of signal amplification. The detection kit and the detection method greatly improve the detection efficiency and sensitivity, so that a detection limit is up to 0.73 pg mL<-1>.

The invention discloses a tobacco mosaic virus (TMV) single colloidal gold quick detection test paper strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a back lining. The colloidal gold pad is coated by a TMV specific antibody marked by colloidal gold. The antibody is secreted by hybridoma cell strain BALB / c-15-50; a detection line and a control line are arranged on the NC membrane, wherein the detection line is coated with specific antigens of three viruses, and the control line is coated with a secondary antibody marked by the colloidal gold. The test paper strip is mainly used for detection on the TMV in tobacco planting regions in Guangdong, is quick, sensitive, accurate and low-cost, has simple operations and achieves simultaneous diagnosis of multiple viruses through one-time sample collection, and is very suitable for in-site primary screening of a large amount of samples. The test paper strip has great application value in practical production and has good promotion and application prospects.

The invention discloses a new strategy enhancing tumor antigen immunogenicity and an application thereof in immunotherapy of lung cancer. In the invention, by means of fusion between a tumor specific antigen and an autophagy gene with high-efficient infection of body APC cells with an adenovirus vector, the synergistic effect of the both can enhance immune response level of human body to a tumor target antigen, thereby achieving a significant antitumor effect.

The invention provides a prostate specific antigen detection kit, detection method and application, and relates to the technical field of antigen detection. The kit and detection method of the present invention use the secondary antibody to modify the DNA, and connect it with the antigen in an adjacent position to form a Y-shaped antigen-antibody-DNA hybrid chain, which is used as a signal converter to convert the antigen input into the DNA signal output; at the same time, the chain substitution reaction Combined with the hybridization chain reaction, the two-stage amplification of the signal is realized; each adjacent two-terminal sequence (hairpin DNAH3 and hairpin DNA H4) on the long-chain DNA assembles to form a magnesium ion DNase, and the enzyme in the presence of magnesium ions The molecular beacon in the cutting solution, the disconnected molecular beacon is released and emits fluorescence, and the new molecular beacon is combined with magnesium ion DNase to be cut, and the fluorescent signal is cyclically amplified to realize the third round of signal amplification, greatly Improved detection efficiency and sensitivity, enabling a detection limit of 0.73pg mL ‑1 .

The invention relates to a new strain of pigs very suitable for xenotransplantation. The first new porcine line lacks a functional porcine endogenous retrovirus and thus is suitable as a donor for tissue and / or cell xenotransplantation into a human recipient. These pigs can also be used as base pigs for further operation, e.g., by gene editing of heteroantigens to produce a second new strain of pigs that is not only free of infectious porcine retroviruses, but also free of major heteroantigens that cause super-acute organ rejection. These pigs can be used to transplant the entire organ, tissue and / or cell into a human recipient. The invention also relates to a method of selecting a pig lacking an infectious porcine endogenous retrovirus, as well as their use for tissue and / or cell xenotransplantation into a human, as well as a method of gene editing of xenoantigens of selected pigs to further enhance donor organs, tissues and / or cells to avoid xenotransplantation rejection.

The invention belongs to the field of biology, and in particular relates to a method for constructing a monkey ITP model induced by a platelet-specific antigen. In particular, it provides a method for obtaining an ITP model by immunizing monkeys with CD61 protein as a specific antigen. Including the application of CD61 protein in the preparation of animal models of thrombocytopenic purpura, the sequence of the CD61 protein is shown in SEQ ID NO:2. The induction method of the invention can effectively induce thrombocytopenia and purpura symptoms, and obtain a stable monkey ITP model.

The invention discloses a TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a piece of water-absorbent filter paper and a backing, wherein a colloidal gold labeled TMV specific antibody, a CMV specific antibody and a PVY specific antibody are enveloped on the colloidal gold pad, and the three antibodies are secreted from hybridoma cell strains BALB / c 15 50, BALBc 15 27 and BALBc 15 8; a detection line and a control line are arranged on the NC membrane; specific antigens of three viruses are enveloped on the detection line; and a colloidal gold labeled second antibody is enveloped on the control line. The test strip is especially suitable for detecting the TMV, CMV and PVY in Guangdong tobacco-growing areas, and the test strip is rapid, sensitive, accurate, low in cost and convenient to operate; the test strip is capable of simultaneously diagnosing various viruses by conducting sampling once; the test strip is quite suitable for in-situ preliminary screening of a great batch of samples; and the test strip, in actual production, has a high application value, and the test strip has a good popularization and application prospect.

The invention relates to isolation, identification and application of a Fabricius bursa active hexapeptide for promoting an immunoreaction of avian influenza and / or a Newcastle disease bivalent vaccine. The Fabricius bursa active hexapeptide has an amino acid composition of Lys Gly Asn Arg Val Tyr, has a simple structure and is extremely weak in immunogenicity. The active peptide has the functionof promoting the immune response of the vaccine and can promote the antibody reaction against the AIV antigen and the antibody reaction against the NDV antigen in the mouse in the mouse immunization experiment. The active peptide can promote the cellular immune response, improve lymphocyte viability and an antigen presentation reaction and improve the vaccine immunopotency, can be used as a vaccine adjuvant or immunopotentiator in animal vaccine application research, can improve the immune response of animal organisms to specific antigens, can improve the immune efficacy of vaccines and can improve the ability of animal organisms resisting epidemic disease infection.

The invention relates to a specific antigen gene VAL28 of clonorchis sinensis and medical application thereof, and the specific antigen gene VAL28 is a novel gene protein. The colloidal gold test strip prepared on the basis of the clonorchis sinensis VAL28 protein has the advantages of high stability, strong specificity, good sensitivity, low detection cost and the like, is convenient to use, simple to operate, simple and clear in detection result and suitable for clinical rapid diagnosis, solves the problems that clinical disease detection and diagnosis time is short, the number of samples to be detected is large and the like, and has good application prospects. Meanwhile, the disease burden caused by clonorchiasis sinensis can be reduced to a certain extent through the good detection method.

The invention relates to a whole blood interferon-gamma (INF-gamma) specific antigen protein prepared by a gene engineering method and a preparation method and application thereof, a nucleotide sequence for coding the protein, a recombinant vector and a recon. The antigen protein is a fusion protein obtained by connecting early secreting antigen target 6 (ESAT-6), cultufiltrate protein 10 (CFP-10) and MPB64 in any order through a connecting peptide, wherein the connecting peptide is a short peptide which does not influence the immunogenicity of the ESAT-6, the CFP-10 and the MPB64. The tuberculosis specific antigen quickly diagnoses tuberculosis, has high sensitivity and specificity, and has great significance for the early diagnosis of the tuberculosis.

The invention discloses a TMV-CMV (Tobacco Mosaic Virus-Cucumber Mosaic Virus) double-virus colloidal gold quick detection test strip which consists of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a back lining, wherein the colloidal gold pad is coated with a TMV specific antibody and a CMV specific antibody which are marked by colloidal gold; the two antibodies are respectively generated by hybridoma cell strains BALB / c-15-50 and BALBc-15-27; a detection line and a control line are arranged on the NC membrane; the detection line is coated with specific antigens of two viruses; the control line is coated with a colloidal gold marked second antibody. The test strip is quick, sensitive, accurate, low in cost and easy and convenient to operate particularly for detection of TMV and CMV in Guangdong tobacco-growing areas; simultaneous diagnosis of the two viruses is realized by one-time sampling; the TMV-CMV double-virus colloidal gold quick detection test strip is very suitable for on-site preliminary screening of a large batch of samples and has application value to actual production and a good popularization and application prospect.