31 results about "Heteroantigens" patented technology

The invention relates to a preparation method of a prostate tumor marker immunosensor. The preparation method comprises the following steps: (1) dissolving silver-hybridized NH2-MCM-48 into a phosphate buffered solution, uniformly dispersing in an ultrasonic mode, further adding a prostate specific antigen second antibody, oscillating at the temperature of 4 DEG C, centrifuging to remove the supernate to obtain a second antibody marker Ag@NH2-MCM-48 / Ab2; (2) firstly, modifying the surface of a glassy carbon electrode as a working electrode by using an amino graphene-ferrocenecarboxaldehyde composite material solution, subsequently modifying by using glutaraldehyde, further modifying by using a prostate specific antigen-anti-solution, dropping a bovine serum albumin solution, continuously modifying prostate specific antigen, and finally modifying Ag@NH2-MCM-48 / Ab1 so as to obtain the prostate tumor marker immunosensor. The immunosensor prepared by using the preparation method is applied to measurement on the prostate specific antigen, and is high in sensitivity and good in specificity.

The invention relates to a diagnostic kit for tuberculosis and mycobacterium tuberculosis infectors, a preparation method and an application method thereof. By using the linker for encoding 15 amino acid (G4S1) 3, the encoding genes (SEQ.ID.NO.6) of a mycobacterium tuberculosis specific antigen Rv3875 and Rv3874 are connected in series, and then inserted into an E. coli expression vector, and the high-efficiency expression and purification for the fusion protein of Rv3875 and Rv3874 in the E. coli are achieved. The recombinant protein at least comprises 8 T cell epipositions which can be used for cell immunity diagnosis, and a diagnostic kit and diagnostic method for a whole blood IFN-Gamma release analysis method are established by taking the protein as the basis and combining the human IFN-Gamma enzyme-linked immunoassay technology, and can be used for the early, specific diagnosis and screening of tuberculosis and mycobacterium tuberculosis infectors.

The invention relates to a joint detection device and a preparation method of helicobacter pylori urease antibodies IgM and IgG. The helicobacter pylori urease antibodies are prepared from a nitrocellulose membrane, a glass fiber adsorbing a colloidal gold labeled helicobacter pylori urease antigen and a mouse IgG, a sample pad, absorbent paper and other auxiliary materials, wherein the materials are adhered together, and the nitrocellulose membrane contains a purified high-specificity mouse-anti-human IgM and IgG antibodies, and a goat-anti-mouse antibody in a solid phase manner. The joint detection device has the advantages that the structure is simple, the conception is novel, the nitrocellulose membrane are coated with the anti-human IgM and IgG antibodies, so that the specificity is strong, and the helicobacter pylori urease antibodies IgM and IgG in a specimen are simultaneously detected without increasing the production operation complexity. Proper gold spray buffer and sample pad treating fluid are matched to effectively improve the reaction sensitivity on the basis of guaranteeing complete release of immunocolloidal gold, and under the same threshold, the use amount of the immunocolloidal gold can be reduced to save the cost. The detection device is high in sensitivity, strong in specificity, simple, convenient and strong in practicability and can realize the time-saving aim during operation.

The present invention concerns a method for prophylactic and / or therapeutic vaccination and / or treatment and / or diagnosis of HIV / AIDS, other infectious diseases, inflammatory and angiogenic diseases and tumours which utilizes a biologically active HIV-1 Tat protein, fragments or derivates thereof, as a module with one or more of the following features: antigen, adjuvant and targeting-delivery system to specific antigen-presenting cells including dendritic cells, endothelial cells and macrophages. In particular, it is claimed that Tat can be used only in its biologically active form as an antigen, alone or combined with one or more other antigens, to prime or to boost protective immune responses against itself as well as other antigens and / or to deliver these antigen(s) as well as active compounds to dendritic cells, endothelial cells and macrophages, due to its capability of targeting these APC and of activating their maturation and functions and of increasing Th-1 type immune responses as an adjuvant. Therefore, due to these characteristics and to the distribution of these cells in the body (during physiological and pathological disorders), biologically active Tat, fragments or derivates thereof containing the RGD region, can be used for preventive, therapeutic and / or diagnostic purposes for HIV / AIDS, other infectious diseases, inflammatory and angiogenic diseases and tumors.

The present invention provides novel vaccines, methods for the production of such vaccines and methods of using such vaccines. The novel vaccines of the present invention combine both of the signals necessary to activate native T-cells-a specific antigen and the co-stimulatory signal-leading to a robust and specific T-cell immune response.

The invention provides a preparation method for a dendritic cell vaccine loaded by a tumor specific antigenic epitope polypeptide. The method comprises the following steps: adopting a human leucocyte antigen A201 positive epitope of an epithelial cell adhesion molecule identified by tumor specific expression, namely a polypeptide composed of amino acid sequences represented by any one of SEQ ID NO.1-6; loading dendritic cells by using the polypeptide of any one of the SEQ ID NO.1-6 to prepare the dendritic cell vaccine. The dendritic cell vaccine can be used for initiating a very strong target anti-tumor cytotoxic T lymphocyte effect. The invention further provides a kit for preparing the dendritic cell vaccine loaded by the tumor specific antigenic epitope polypeptide.

The invention discloses an enzyme-linked immunospot test method for T lymphocytes. The method comprises the following steps: by adopting a T-SPOT.TB kit produced in British Oxford Immunotec Company, taking 5ml of sodium citrate anticoagulation venous blood, separating mononuclear cells of peripheral blood and placing the mononuclear cells on a gamma-interferon antibody coated porous plate, adding an early secretory antigenic target (ESAT-6) of tuberculosis specific antigen and a culture filtrate protein (CFP-10) to serve as stimulus antigens, culturing in an incubator for 24 hours, washing off T lymphocytes with antigen sensitization effects, adding a biotin labeled second antibody, performing enzyme linked colorimetry, forming spots at secreting positions of cell factors, and detecting the number of the T lymphocytes with the tuberculosis specific antigen sensitization effects by recording the spot number. The method disclosed by the invention contributes to early screening of culture negative pulmonary tuberculosis.

The invention discloses a colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY). The test strip consists of a sample pad, a colloidal gold pad, an NC film, a piece of water absorption filter paper and a back lining, wherein the colloidal gold pad is enveloped with a TMV specific antibody and a PVY specific antibody which are marked by colloidal gold, and the TMV specific antibody and the PVY specific antibody are respectively generated by secretion by hybridoma cell lines, i.e., BALB / c-15-50 and BALB / c-15-8; the NC film is provided with a detection line and a control line; the detection line is enveloped with specific antigens of the TMV and the PVY; the control line is enveloped with a second antibody marked by the colloidal gold. The rapid detection colloidal gold test strip is especially suitable for the detection on the TMV and the PVY in Guangdong tobacco-growing areas, and is rapid, sensitive and accurate in dection, low in cost and simple and convenient in operation; the test strip can be used to diagnose the two viruses at the same time by one-time sampling, thus being very suitable for field primary screening of a great batch of samples; therefore, the colloidal gold test strip has a good application value in actual production and is good in popularization and application prospects.

The invention provides a detection kit for a prostate specific antigen, a detection method and an application thereof, which relate to the technical field of antigen detection. The kit and the detection method of the invention utilize a secondary antibody to modify DNA, form a Y-shaped antigen-antibody-DNA hybridization chain by means of proximity ligation with the antigen, and converts the antigen input into DNA signal output by using the antigen-antibody-DNA hybridization chain as a signal converter; meanwhile, a chain substitution reaction is combined with a hybridization chain type reaction, and two-stage amplification of the signal is achieved; and every two adjacent two-end sequences (hairpin DNA H3 and hairpin DNA H4) on long-chain DNA are assembled to form a magnesium ion DNA enzyme, the magnesium ion DNA enzyme cuts molecular beacons in a solution in the presence of magnesium ions, the cut-off molecular beacons are released and fluoresce, new molecular beacons are combined with the magnesium DNA enzyme and cut off by the same, and a fluorescent signal is cyclically amplified to realize the third round of signal amplification. The detection kit and the detection method greatly improve the detection efficiency and sensitivity, so that a detection limit is up to 0.73 pg mL<-1>.

The invention discloses a colloidal gold test strip for rapidly detecting CMV-PVY double viruses. The colloidal gold test strip is composed of a sample pad, a colloidal gold pad, an NC film, water-absorbing filter paper and a back lining, wherein the colloidal gold pad is coated by a CMV specific antibody and a PVY specific antibody labeled with colloidal gold, and the two antibodies are secreted by hybridoma cell strains BALBc-15-27 and BALBc-15-8; the NC film is provided with a detection line and a control line, and the detection line is coated by specific antigens of two viruses; the control line is coated with second antibodies labeled with colloidal gold. The test strip is particularly suitable for detecting CMV and PVY in the Guangdong tobacco-growing areas, is rapid, sensitive and accurate, is low in cost, and is easy and convenient to operate, the purpose that the two viruses are simultaneously diagnosed through once sampling is realized, and the test strip is particularly suitable for carrying out on-site preliminary screening on large-batch samples, has an application value in the practical production, and has good popularization and application prospects.

The present invention relates to a medicine for diagnosing carcinoma of stomach and its preparation method. Said medicine includes antibody and radioactive nuclide, the descried antibody is monoclonal antibody 3H11, and the described radioactive nuclide can utilize a bifunctional connecting agent to mark the described monoclonal antibody 3H11, and the monoclonal antibody marked by the described radioactive nuclide is a colourless transparent liquid injection. Because the tumor cell surface can express specific antigen, so that it can utilize the specific combination of antibody and antigen, and utilize said antibody to make the radioactive nuclide be loaded on the tumor region, and utilize nuclear medicine image technique to make diagnosis and location of tumor.

The invention discloses a tobacco mosaic virus (TMV) single colloidal gold quick detection test paper strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a back lining. The colloidal gold pad is coated by a TMV specific antibody marked by colloidal gold. The antibody is secreted by hybridoma cell strain BALB / c-15-50; a detection line and a control line are arranged on the NC membrane, wherein the detection line is coated with specific antigens of three viruses, and the control line is coated with a secondary antibody marked by the colloidal gold. The test paper strip is mainly used for detection on the TMV in tobacco planting regions in Guangdong, is quick, sensitive, accurate and low-cost, has simple operations and achieves simultaneous diagnosis of multiple viruses through one-time sample collection, and is very suitable for in-site primary screening of a large amount of samples. The test paper strip has great application value in practical production and has good promotion and application prospects.

The invention relates to the medicinal field, and more specifically relates to an application of a monoclonal antibody of an anti-epidermal growth factor receptor in the aspect of treating colorectal cancer, in particular to a humanized monoclonal antibody-nimotuzumab. The monoclonal antibody is a specific purified antibody generated aiming at a specific antigen, has high specificity, can specifically carry out targeted therapy aiming at tumor cells, can reverse the malignant biological behavior of the tumor cells at the molecular level, has the characteristics of strong targeting property, high specificity, low toxic side effect and the like, and can obviously enhance the therapeutic effects of radiotherapy and chemotherapy.

The present invention relates to a medicine for curing carcinoma of stomach and its preparation method. Said medicine includes antibody and radioactive nuclide. Said antibody is monoclonal antibody 3H11, said radioactive nuclide can utilize a bifunctional connecting agent to mark the described monoclonal antibody 3H11. Because the tumor cell surface can express specific antigen, so that it can utilize the specific combination of antibody and antigen, and can utilize antibody to make the radioactive nuclide be loaded on the tumor region, and utilize beta-particles released by radioactive nuclide decay to kill carcinoma cell so as to attain the goal of curing carcinoma.

The invention relates to minicircle DNA vaccine design and application. Specifically, the invention relates to a minicircle DNA vector for expressing specific antigens or antigen fragments of pathogenic microorganisms in vivo. The minicircle DNA vector can mediate antigen proteins to be efficiently expressed in vivo, the immunogenicity of DNA vaccines is enhanced, and meanwhile, the safety problemcaused by resistance genes can be avoided. The minicircle DNA vector can be used for preventing and / or treating common infectious diseases and related cancers, and has the advantages of stronger antigen expression and higher safety.

The invention discloses a new strategy enhancing tumor antigen immunogenicity and an application thereof in immunotherapy of lung cancer. In the invention, by means of fusion between a tumor specific antigen and an autophagy gene with high-efficient infection of body APC cells with an adenovirus vector, the synergistic effect of the both can enhance immune response level of human body to a tumor target antigen, thereby achieving a significant antitumor effect.

This disclosure relates to systems and methods that identify, predict, and rank immunogenic T-cell epitopes. In particular, this disclosure identifies epitopes that arose from disease-associated mutations, wherein the epitopes are predicted to elicit immune response from T cells. Specifically, this disclosure simultaneously considers peptide-level information, including MHC Class I and II presentation, helper and cytotoxic T cell response and sample-level information, including mutation clonality and MHC allele dosage. In some embodiment, the systems and methods are used for personalized treatment of cancers.

The invention belongs to the field of biology, and particularly relates to a construction method of a platelet specific antigen induced monkey ITP model. Particularly, the invention provides the method for obtaining the monkey ITP model by taking CD61 protein as a specific antigen for immunization through induction. The method comprises application of the CD61 protein in preparation of the ITP animal model, and the sequence of the CD61 protein is shown as SEQ ID NO: 2. The induction method can effectively induce thrombocytopenia and purpura symptoms, and the stable monkey ITP model is obtained.

The invention discloses a chromatographic strip used for diagnosis of prostate cancer. The chromatographic strip comprises a bottom plate; a sample pad, a conjugate pad, a blood insulation layer, a chromatographic pad and a water absorption pad are sequentially arranged on the bottom plate in an overlapping manner; a magnetic nanometer immunoprobe coupled with an anti-total prostate cancer specific antigen-antibody is arranged on the conjugate pad; a first detecting line, a second detecting line and a quality control line are sequentially arranged on the chromatographic pad; a monoclonal antibody with anti-free prostate cancer specific antigen is arranged in the first detecting line, PSA(prostate-specific antigen)-antibody combined with anti-Alpha-1 antichymotrypsin (ACT) is arranged on the second detecting line, and IgG antibody capable of being combined with total PSA antibody is arranged on the quality control line. The invention further provides a preparation method of the strip. According to the invention, the occurrence of the problem of incapability of determining the ratio of two antigens, caused by partial overlapping of detecting sites between free PSA and total PSA whichare preset on the detecting line originally, is greatly reduced, and the accuracy of the detecting results are improved.

The invention provides a prostate specific antigen detection kit, detection method and application, and relates to the technical field of antigen detection. The kit and detection method of the present invention use the secondary antibody to modify the DNA, and connect it with the antigen in an adjacent position to form a Y-shaped antigen-antibody-DNA hybrid chain, which is used as a signal converter to convert the antigen input into the DNA signal output; at the same time, the chain substitution reaction Combined with the hybridization chain reaction, the two-stage amplification of the signal is realized; each adjacent two-terminal sequence (hairpin DNAH3 and hairpin DNA H4) on the long-chain DNA assembles to form a magnesium ion DNase, and the enzyme in the presence of magnesium ions The molecular beacon in the cutting solution, the disconnected molecular beacon is released and emits fluorescence, and the new molecular beacon is combined with magnesium ion DNase to be cut, and the fluorescent signal is cyclically amplified to realize the third round of signal amplification, greatly Improved detection efficiency and sensitivity, enabling a detection limit of 0.73pg mL ‑1 .

The invention belongs to the field of biology, and in particular relates to a method for constructing a monkey ITP model induced by a platelet-specific antigen. In particular, it provides a method for obtaining an ITP model by immunizing monkeys with CD61 protein as a specific antigen. Including the application of CD61 protein in the preparation of animal models of thrombocytopenic purpura, the sequence of the CD61 protein is shown in SEQ ID NO:2. The induction method of the invention can effectively induce thrombocytopenia and purpura symptoms, and obtain a stable monkey ITP model.

The invention discloses a TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a piece of water-absorbent filter paper and a backing, wherein a colloidal gold labeled TMV specific antibody, a CMV specific antibody and a PVY specific antibody are enveloped on the colloidal gold pad, and the three antibodies are secreted from hybridoma cell strains BALB / c 15 50, BALBc 15 27 and BALBc 15 8; a detection line and a control line are arranged on the NC membrane; specific antigens of three viruses are enveloped on the detection line; and a colloidal gold labeled second antibody is enveloped on the control line. The test strip is especially suitable for detecting the TMV, CMV and PVY in Guangdong tobacco-growing areas, and the test strip is rapid, sensitive, accurate, low in cost and convenient to operate; the test strip is capable of simultaneously diagnosing various viruses by conducting sampling once; the test strip is quite suitable for in-situ preliminary screening of a great batch of samples; and the test strip, in actual production, has a high application value, and the test strip has a good popularization and application prospect.

The invention relates to isolation, identification and application of a Fabricius bursa active hexapeptide for promoting an immunoreaction of avian influenza and / or a Newcastle disease bivalent vaccine. The Fabricius bursa active hexapeptide has an amino acid composition of Lys Gly Asn Arg Val Tyr, has a simple structure and is extremely weak in immunogenicity. The active peptide has the functionof promoting the immune response of the vaccine and can promote the antibody reaction against the AIV antigen and the antibody reaction against the NDV antigen in the mouse in the mouse immunization experiment. The active peptide can promote the cellular immune response, improve lymphocyte viability and an antigen presentation reaction and improve the vaccine immunopotency, can be used as a vaccine adjuvant or immunopotentiator in animal vaccine application research, can improve the immune response of animal organisms to specific antigens, can improve the immune efficacy of vaccines and can improve the ability of animal organisms resisting epidemic disease infection.

According to the invention, a human serum albumin mature peptide amino acid sequence is analyzed by a computer, and is compared with mouse, cow, monkey, sheep and rabbit albumin mature peptide amino acid sequences to select a plurality of human serum albumin specific antigenic determinant amino acid sequence short peptides, wherein the amino acid lengths of the short peptides are respectively 15-35; an artificial synthetic oligopeptide is used for immunizing mice to obtain mouse hybridoma cell engineering strains capable of generating specific anti-human serum albumin monoclonal antibodies which do not have immune reaction with mice, cattle, monkeys, sheep and rabbits; and the antibody prepared from the cell strain has ultrahigh sensitivity and ultrahigh specificity to human serum albumin.

The invention relates to a specific antigen gene VAL28 of clonorchis sinensis and medical application thereof, and the specific antigen gene VAL28 is a novel gene protein. The colloidal gold test strip prepared on the basis of the clonorchis sinensis VAL28 protein has the advantages of high stability, strong specificity, good sensitivity, low detection cost and the like, is convenient to use, simple to operate, simple and clear in detection result and suitable for clinical rapid diagnosis, solves the problems that clinical disease detection and diagnosis time is short, the number of samples to be detected is large and the like, and has good application prospects. Meanwhile, the disease burden caused by clonorchiasis sinensis can be reduced to a certain extent through the good detection method.

The invention relates to a bursa active pentapeptide for promoting the immune response of AIV and / or NDV vaccines and its application. The bursa active pentapeptide of the present invention has the amino acid composition of Met Pro Pro Thr His, simple structure and extremely weak immunogenicity. The active peptide of the present invention has the function of promoting vaccine immune response, and in the mouse immunization experiment, it not only promotes the generation of AIV-specific antibody response in immunized mice, but also promotes the generation of NDV-specific antibody response in immunized mice. In addition, it also promotes cellular immune response, antigen presentation response, and improves the vaccine immune efficacy of immunized chickens. It can be used as a vaccine adjuvant or immune enhancer in animal vaccine application research to improve the animal body’s immune response to specific antigens. Improve the immune efficacy of the vaccine, thereby improving the ability of the animal body to resist epidemic infections.

The invention discloses a TMV-CMV (Tobacco Mosaic Virus-Cucumber Mosaic Virus) double-virus colloidal gold quick detection test strip which consists of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a back lining, wherein the colloidal gold pad is coated with a TMV specific antibody and a CMV specific antibody which are marked by colloidal gold; the two antibodies are respectively generated by hybridoma cell strains BALB / c-15-50 and BALBc-15-27; a detection line and a control line are arranged on the NC membrane; the detection line is coated with specific antigens of two viruses; the control line is coated with a colloidal gold marked second antibody. The test strip is quick, sensitive, accurate, low in cost and easy and convenient to operate particularly for detection of TMV and CMV in Guangdong tobacco-growing areas; simultaneous diagnosis of the two viruses is realized by one-time sampling; the TMV-CMV double-virus colloidal gold quick detection test strip is very suitable for on-site preliminary screening of a large batch of samples and has application value to actual production and a good popularization and application prospect.

The invention discloses a TMV-CMV (Tobacco Mosaic Virus-Cucumber Mosaic Virus) double-virus colloidal gold quick detection test strip which consists of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a back lining, wherein the colloidal gold pad is coated with a TMV specific antibody and a CMV specific antibody which are marked by colloidal gold; the two antibodies are respectively generated by hybridoma cell strains BALB / c-15-50 and BALBc-15-27; a detection line and a control line are arranged on the NC membrane; the detection line is coated with specific antigens of two viruses; the control line is coated with a colloidal gold marked second antibody. The test strip is quick, sensitive, accurate, low in cost and easy and convenient to operate particularly for detection of TMV and CMV in Guangdong tobacco-growing areas; simultaneous diagnosis of the two viruses is realized by one-time sampling; the TMV-CMV double-virus colloidal gold quick detection test strip is very suitable for on-site preliminary screening of a large batch of samples and has application value to actual production and a good popularization and application prospect.