The invention discloses a preparation technology of a rice wine capable of nourishing yin and tonifying kidneys. The rice wine is prepared from the following raw materials in parts by weight: 8-11 parts of polished nutrient powder of edible mushrooms, 3-8 parts of Chinese yam, 1-5 parts of polygonatum odoratum, 2-8 parts of polygonum multiflorum, 1-7 parts of wolfberry, 2-9 parts of angelica sinensis, 3-9 parts of ginkgo leaves, 1-8 parts of mint, 5-10 parts of beef liver, 5-15 parts of white chrysanthemum, 5-15 parts of radix angelicae, 5-15 parts of radix paeoniae alba, 5-15 parts of white poria, 5-15 parts of ginkgo, 400-500 parts of zinc-rich rice, 10-15 parts of jade beauty, 30-40 parts of black tomatoes, 20-30 parts of longan, 10-15 parts of chestnut kernel, 10-15 parts of walnut kernel, 5-6 parts of herba epimedii, 3-4 parts of pyrola corbieri, 1-2 parts of flos caryophylli and 7-8 parts of balsam pear seeds. The rice wine is abundant in nutrient, clear in juice, mellow in mouthfeel, simple and elegant in aroma and long in aftertaste, and has the efficacies of nourishing yin, tonifying the kidneys, losing weight, beautifying the features, tonifying yang and qi, tonifying the heart and spleen, nourishing the blood for tranquilization and relaxing bowel, and is suitable for most of people for drinking.