The invention discloses biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 50 to 60 parts of livestock and poultry manure, 10 to 15 parts of humic acid, 25 to 30 parts of saw dust, 5 to 8 parts of alkaline residue, 10 to 15 parts of aggregate agent, 8 to 10 parts of complex microbial agent, 5 to 8 parts of water retainingagent and 5 to 11.5 parts of agricultural waste. The biological organic fertilizer disclosed by the invention is capable of effectively reducing or solving the situation of soil acidification, meanwhile, an aggregate structure can be generated by soil, the porosity of the aggregate structure can be larger than that of a simple-grain soil structure under the situation that the bulkiness of the soilis ensured, water-through and air-permeable effects are better, the rooting effect of plants can also be better, and good growth of the plant can be ensured; the complex microbial agent is capable ofpurifying harmful substances in the soil, the biochemical degree is high, and the health degree of crops is increased.