The invention aims to provide a multistage resistive random access memory and a manufacturing method of the multistage resistive random access memory. The multistage resistive random access memory comprises a substrate, a bottom electrode, a resistive layer and a top electrode, wherein the substrate, the bottom electrode, the resistive layer and the top electrode are arranged from bottom to top in sequence. The multistage resistive random access memory is characterized in that an isolation layer is further arranged between the bottom electrode and the resistive layer. According to the multistage resistive random access memory and the manufacturing method of the multistage resistive random access memory, the nanoscale isolation layer is additionally arranged, so that the magnitude of a storage window of the resistive random access memory is increased to over 105, and the primary condition needed by multistage storage is met; electrochemical active materials are used as the top electrode, different resistance states are achieved under the condition of different voltage drives through the drifting character of the electrochemical active materials, and the purpose of multistage storage is achieved; in addition, due to the additional arrangement of the isolation layer, dissipation of oxygen ions in the movement process is reduced, the bottom electrode is effectively protected, and the stability of a device is improved. In addition, the manufacturing method of the multistage resistive random access memory is simple and easy to control.