The invention belongs to the technical field of the conservation of horticultural plants, the technical deficiencies of the technique of hole punching in the tree trunk in the infusion process of the tree trunk, the high reliance on electricity in the prior art, the increase in the number of wounds on the trunk skin and the like are fixed. In particular, a tree trunk punching machine and an application thereof are provided. The punching machine comprises a machine frame, a transmission device and a fixing device. When the punching machine is in use, an axle sleeve is firstly fixed well on the machine frame, a drill is fixed on a transmission shaft, then the transmission shaft is encased in the axle sleeve, fixed holes are threaded with rope stripes, the punching machine is fixed on the main trunk, the control feet are wrung, after the angle of hole is adjusted well, the rope stripes are buckled tightly, the crank hand is turned, the punching work is conducted, after the impelled depth is fit, the crank hand is reversely turned, and the drill is backed out of the main trunk, and the punching work of the main injection hole is completed easily. When the tree trunk punching machine is used, electricity is not required; the tree trunk punching machine is widely applied in the tree trunk transfusion of big tree transplanting in landscaping, fruit tree pest control and the like, the machine is simple in structure, flexible in operation, and easy to promote.