The invention discloses a broad bean red spot pathogenic bacterium laboratory
spore production method, and belongs to the field of
plant protection. The method comprises the following two steps: firstly, carrying out low-
temperature stress treatment, putting a sterilized wet carrier into a culture dish, then putting
hypha blocks or mature sclerotia of
pathogenic bacteria of the red spot
disease of the
broad beans, freezing for 0.5-1 day in a dark place at the temperature of-25 DEG C to-10 DEG C, and then culturing for 3-5 days at the constant temperature of 15-25 DEG C; and then carrying out variable-temperature alternate induction culture, alternately culturing the treated material under the constant-temperature conditions of 9-19 DEG C and 2-8 DEG C for 12 hours, culturing
hypha blocks for 5-7 days, and culturing mature sclerotia for 9-11 days until conidiophores and conidia can be seen by naked eyes. The
hypha blocks or
sclerotium are prepared by activation culture of a PDA culture medium, and the
wetting carrier is
filter paper, gauze or
sponge which is soaked with sterile
distilled water after sterilization. Efficient and stable
spore production is achieved through low-
temperature stress treatment and variable-temperature alternate induction culture, a key material is provided for related scientific research and green prevention and control of the broad bean red spot
disease, and the method has important practical value.