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42results about How to "Simple and flexible way" patented technology

Lysozyme with covalent modification through medium and long chain fat chain acyl-free sophorolipid derivatives and modifying method and application of lysozyme

The invention discloses a lysozyme with covalent modification through medium and long fat chain acyl-free sophorolipid derivatives (DSL-C12) and a modifying method and application of the lysozyme. The modifying method comprises the following steps that firstly, active esters of sophorolipid derivatives are dissolved in DMSO, then a NaHCO3 aqueous solution of lysozyme is added, a condensation reaction is carried out under the stirring condition, and a reaction mixture is obtained after the reaction is finished, wherein the sophorolipid derivatives are carboxylic acid type medium and long fat chain sophorolipid; secondly, deionized water and a phosphate buffer solution are adopted in sequence to carry out dialysis on the mixture obtained in the first step, obtained dialysate is subjected to centrifugation separation, and the modified lysozyme is obtained. The modified lysozyme can effectively suppress the activity of gram-positive bacteria and can also remarkably suppress the activity of gram-negative bacteria, so that the antibacterial spectrum of natural lysozyme is expanded, and the lysozyme can serve as a multifunctional food preservative.
Owner:ZHEJIANG GONGSHANG UNIVERSITY

Rapid table refreshing method based on hardware DMA

PendingCN114679381ASolve the shortcoming of long power-on initialization timeQuick refreshTransmissionEnergy efficient computingDatasheetDirect memory access
The invention relates to the related technical field of network exchange, in particular to a rapid table refreshing method based on hardware DMA (direct memory access), which comprises the following steps: firstly, constructing a descriptor space and a data space in a memory, filling descriptor table items in the descriptor space, filling data table items in the data space in a message manner, and storing information of the data table items in the descriptor table items; then informing the DMA of the index address of the descriptor table item, initiating a read operation by the DAM through the index address to obtain the descriptor table item, and extracting a corresponding data table item message according to information in the descriptor table item; and finally, analyzing the data table item message to obtain configuration information, recombining the configuration information, and writing the recombined configuration information into a corresponding table item RAM (Random Access Memory) through a configuration interface to complete table item configuration. According to the method and the device, the burden of a CPU (Central Processing Unit) can be reduced, table entries with any bit width of 64-512 bits can be flexibly configured through setting, and the table refreshing time is greatly saved due to the adoption of a pure hardware mode.
Owner:芯河半导体科技(无锡)有限公司

A kind of lysozyme covalently modified by short aliphatic chain sophorolipid derivatives and its modification method and application

The invention discloses lysozyme covalently modified by carboxylic acid type short-aliphatic-chain sophorolipid derivatives as well as a modification method and an application of lysozyme. The modification method comprises the following steps: (1) dissolving active esters of sophorolipid derivatives in DMSO (dimethyl sulfoxide), then adding the dissolved active esters in a NaHCO3 water solution of the lysozyme, and conducting a condensation reaction under a stirring condition, so that a reaction mixture is obtained after the reaction, wherein the sophorolipid derivatives are carboxylic acid type short-aliphatic-chain sophorolipid derivatives; and (2) dialyzing a buffer solution system obtained in the step (1) sequentially by virtue of de-ionized water and a phosphate buffer solution, and conducting centrifugal separation on an obtained dialysate, so that the modified lysozyme is obtained. The modified lysozyme not only can effectively inhibit the activity of Gram-positive bacteria but also can significantly inhibit the activity of Gram-negative bacteria; therefore, the antibacterial spectrum of natural lysozyme is expanded; and the lysozyme can serve as a multifunctional food preservative.
Owner:ZHEJIANG GONGSHANG UNIVERSITY
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