The invention relates to a multi-sensor arrangement device for temperature and humidity detection and calibration of a constant-temperature constant-humidity box, a stand column is arranged on a base,a supporting seat is arranged on the stand column in a penetrating mode, the supporting seat can slide up and down along the stand column, the supporting seat fixedly connected with the stand columnby a jackscrew, a plurality of rotating shafts are arranged on the supporting seat, one ends of cantilevers sleeve the rotating shafts, the cantilevers can rotate horizontally relative to the rotatingshafts, the cantilevers can be fixedly connected with the rotating shafts by jackscrews, the cantilevers are telescopic cantilevers, the other ends of the cantilevers are hinged to one ends of extension arms, the extension arms are telescopic extension arms, the other ends of the extension arms are hinged to one ends of connection blocks, the other ends of the connection blocks are hinged to sensor supports, and mounting holes used for mounting temperature sensors and humidity sensors are formed in the sensor supports; multi-layer multi-point arranged sensors can be arranged in the constant-temperature constant-humidity box at one time, the rapid arrangement of the sensors can be realized when the constant-temperature and constant-humidity box is calibrated, the calibration efficiency canbe effectively improved, the labor intensity is reduced, and the accurate calibration result is ensured.