The invention discloses a highway real-time traffic accident risk assessment method based on deep learning, and the method comprises the steps: firstly dividing a highway into road segments through employing the basic information of an ETC portal, highway intercommunication and a toll station, and building the upstream and downstream association relation between the road segments; calculating thetraffic flow, the traffic flow speed and the traffic flow density of each road section respectively, obtaining road information, meteorological information and accident information, converting the road information, the meteorological information and the accident information into one-hot codes, and then performing data fusion, data resampling and standardization on four types of information corresponding to upstream and downstream road sections of an accident occurrence point; distinguishing time sequence features and non-time sequence features according to the acquired data, and constructing and training a deep learning model; and finally, according to the trained deep learning model, carrying out real-time evaluation on the risk level of the traffic accident of each road section of the expressway, and calculating to obtain an accident risk level index. According to the invention, the highway traffic accident risk level can be evaluated timely and accurately.