35 results about "Valine methyl ester" patented technology

The invention discloses a production method of alpha-amino acid ester acyltransferase and valine dipeptide, and relates to the technical field of biology. The amino acid sequence of the alpha-amino acid ester acyltransferase is as shown in SEQ ID NO. 4. Alpha is modified through design; according to the present invention, the mutant with the significantly improved catalytic performance is successfully obtained, and the efficient and green enzyme-method valine dipeptide synthesis process is established. The process is carried out under mild conditions, L-valine methyl ester and L-tryptophan are taken as substrates, and high-activity mutant enzyme expressed by engineering bacteria is utilized for catalysis, so that the yield of the valine-tryptophan dipeptide is up to 22.42 g / L, which is 1.62 times that of original enzyme, and the defects of traditional chemical synthesis and existing enzymatic process are effectively overcome; the method has the outstanding advantages of simple steps, mild conditions, high product concentration, environmental friendliness and the like, and provides a reliable technical scheme with wide prospects for industrial production of valine dipeptide.