The invention relates to a method for detecting antibiotic residues in meat food, and relates to the field of meat food detection. The method comprises the following steps of: adding meat food to be detected into a stirring pulverizer for pulverization, adding an extractant, soaking for 30-60min, grinding the mixture into fine powder via a grinding miller after performing freeze drying, adding a cosolvent into the fine powder, performing wall breaking by using a wall breaking machine, soaking at 40-50 DEG C for 60-100min, respectively adding 1mL of mixed solution in a reagent bottle in the second step to 6 glass test tubes, adding 10mL of culture solution into each glass test tube, adjusting the pH of each glass test tube to neutrality, adding bacterial spores to each glass test tube, performing oscillation and uniform mixing, stewing for 1-2d, and determining whether the antibiotic residues in meat food meet the requirements through the color, the absorbancy and the number of bacterial spores of the solution in each glass test tube. By adopting the detection method, by observing the color change, the absorbancy and the number of bacterial spores of the solution, and mutually comparing the three kinds of data, the detection is more accurate, and potential safety hazards of the food caused by inaccurate detection data are avoided.