The invention provides a bi-radical compound composed of a triaryl methyl radical and a nitroxide radical and salt thereof and a preparation method and application of the bi-radical compound composedof the triaryl methyl radical and the nitroxide radical and the salt thereof. A structural formula of the bi-radical compound is as shown in the formula 1; the formula is shown in the description; thepreparation method comprises the steps that a compound A1 and a compound A2 are subjected to acid amide condensation reaction to obtain a compound A3; the compound A3 and a compound A4 are subjectedto acid amide condensation reaction to obtain the bi-radical compound and the salt thereof. The portion, provided with both the nitroxide radical and the triaryl methyl radical, of a bi-radical polarization agent has the following advantages that the frequency matching ability is better, and the CE efficiency is higher; under a magic angle spinning condition, a depolarization effect does not exist, and a high field DNP enhancing effect is better; exchange interaction is stronger; the water solubility is good, so that the DNP enhancing multiple is high; by modifying R1, R2, R3, R4, R5 and R6 groups, exchange interaction can be improved moderately, bi-radical relaxation time can be prolonged moderately, and the bi-radical water solubility can be improved moderately, so that the DNP propertyof the polarization agent is improved.