The invention discloses a nitrogen-rich microalloy fine grain HRB400E straight aseismic steel bar and a preparation method thereof. The steel bar is prepared from the following chemical components in parts by weight: 0.21 to 0.25 weight percent of C, 0.42 to 0.55 weight percent of Si, 1.38 to 1.50 weight percent of Mn, 0.012 to 0.020 weight percent of V, less than or equal to 0.045 weight percent of S, less than or equal to 0.045 weight percent of P, 0.0090 to 0.0115 weight percent of N and the balance of Fe and inevitable impurities. The steel bar is prepared through the following steps of molten steel smelting, deoxidation alloying, molten steel argon station refining, molten steel casting, steel billet heating and steel billet controlled rolling and controlled cooling in sequence. The process disclosed by the invention has the characteristics of low production cost, high process applicability, high controllability and the like, and various strengthening effects such as precipitation strengthening, fine grain strengthening and the like are fully exerted by integrating and innovating chemical component design, converter smelting, a deoxidation alloying structure, continuous casting, a steel rolling heating schedule, rolling temperature and a controlled cooling process; and the produced reinforcing steel bar has the advantages of excellent and stable process mechanical properties, fine and uniform microscopic structure, good plasticity and toughness, excellent anti-seismic property and the like.