The invention belongs to the technical field of food processing, and discloses a soup base composition of beef broth. The soup base composition comprises beef, beef calf bone, beef tripe, beef sausage, beef liver, beef suet, vermicelli, tremella, tofu skin, mung bean cake, seasoning packet, green onion, garlic seedling, coriander, chicken essence, chili sauce, medlar, ice sugar, ginger, salt and monosodium glutamate and the thirteen kinds of spices. The invention also discloses a preparation method of the soup bases composition of beef broth, the finished beef broth is obtained through the steps of main ingredient cleaning, ingredients preparing, beef broth boiling and finished beef broth preparation. The amount of each raw material in the soup bases composition is fixed, the raw materialscan be boiled only by weighing the raw materials according to the amount in the formula in the process of beef broth boiling, and less errors are made. The preparation method further guarantees the quality of the finished beef broth by optimizing the amount of raw materials, the adding time, the adding sequence and the using method, and better avoids the influence of human factors on the finishedbeef broth.