The invention discloses an operation method for safely applying a line protection device to a wind power station, which relates to the relay protection of a power plant and is provided aiming at solving the problem that the line protection device in the current system cannot meet the protective requirements of the wind power station very well. The operation method comprises the following steps of: firstly, establishing a real-time simulation model which is a virtual power grid environment, including fault points in a wind power station, a bus and transmission lines, a main transformer unit of a power generator, a current and voltage mutual inductor, an equivalent system and a simulation system; secondly; establishing an experimental detection platform including a real-time digital simulation work station, an RTDS (Real Time Digital System) real-time digital simulator, an analogue quantity output board card, a power amplifier, a line protection device, an analogue circuit breaker, a switch conversion device and a digital quantity input board card; and thirdly, accessing the line protection device to be detected to the power amplifier of the detection system, then connecting the line protection device with the analogue circuit breaker and carrying out various kinds of experiments. The experiment implemented by using the wind power station model and an onsite oscillograph of the wind power station can provide a real and accurate simulation environment so that validation possibility is improved, and therefore the line protection device to be detected can be safely used for the wind power station after detected.