The invention, which relates to the space optical remote sensing field, provides an alignment system and adjusting method for a large-calibre space optical camera lens. With the system and method, problems of low optical transfer function and imaging quality of the optical system of the existing large-calibre space optical camera lens can be solved. The alignment system comprises a camera lens, a lens alignment frame, three lens connecting bases, a secondary lens alignment frame, a secondary lens adjusting frame, a plane lens, a plane lens two-dimensional adjusting mechanism, an interferometer, an interferometer adjusting frame, and a three-lens adjusting frame. The camera lens consists of a camera frame, a secondary lens assembly, a secondary lens pad block, a main lens assembly, a camera substrate, a rear bracket, a first folding lens assembly, a three-lens assembly, a three-lens pad block, a second folding lens assembly, and three connecting supports. With the alignment structure at the vertical direction of the optical axis of the camera lens, the precision changes of relative positions of all reflection lenses are small and the imaging quality of the optical system is kept to be the same when the large-calibre space optical camera lens enters a microgravity environment of the space after alignment on the ground.