The invention discloses a collophanite reverse flotation collector and a preparation method and an application method thereof, and belongs to the technical field of solid mineral resource separation. Vacuum five-line phenol extract oil, a diluent, fuming sulphuric acid, sodium hydroxide and the like are used as raw materials, after raw oil and the diluent are mixed according to a certain mass percent, the fuming sulphuric acid is fed into a reaction still for sulfonation, stirring and washing are conducted after a sulfonation reaction is completed, still standing is conducted for layering, an organic layer is taken for primary saponification in a saponification reaction still, and sulphonate is obtained; and the obtained sulphonate and soy acid are compounded into a mixture according to a certain mass percent, the mixture is subjected to secondary saponification, and the anionic high-calcium (high-magnesium) collophanite reverse flotation collector is obtained. The collector is simple in preparation process, high in adaptability (such as the temperature and the ore pulp pH), good in mud resistance, high and stable in flotation index, low in cost and free of toxicity; and when the collector is applied to high-calcium (high-magnesium) collophanite flotation, the high-phosphate concentrate grade (P2O5) can be remarkably improved, the recovery rate of high-phosphate concentrate can be remarkably increased, and the impurity content of the concentrate is lowered.