The invention relates to the technical field of endoscopes, in particular to an endoscope self-locking device. The device comprises a shell and a rotating handle, a first locking piece is fixedly arranged in the shell, a rotating shaft is rotatably connected into the first locking piece, and a locking mechanism used for being matched with the first locking piece is movably connected to the rotating shaft; the locking mechanism comprises an operating rod, a rolling wheel, a locking piece, a wedge-shaped block and a second locking piece, the locking piece is connected to the operating rod, the operating rod is matched with the wedge-shaped block, the wedge-shaped block is arranged on the second locking piece, the second locking piece is movably connected to the rotating shaft, and the secondlocking piece can only move in the axial direction of the rotating shaft; and the operating rod controls the second locking piece to be matched with the first locking piece to achieve self-locking, and meanwhile the locking piece locks the operating rod. Self-locking can be achieved by rotating the handle, locking of an insertion tube is achieved, the imaging stability of the insertion tube is guaranteed, and meanwhile the fatigue strength of an operator is reduced.