The invention provides application of a traditional Chinese medicine composition prepared from processed radix aconiti carmichaeli, rhizoma zingiberis, radix glycyrrhizae preparata, poria cocos, bighead atractylodes rhizome, radix ginseng rubra, white paeony root, fructus corni, fructus amomi, herba ephedrae, herba asari, radix angelicae sinensis, rhizoma cimicifugae and radix puerariae to preparation of medicine for regulating intestinal flora balance. The invention further provides application of traditional Chinese medicine extract, which is prepared by decocting and fermenting the traditional Chinese medicine composition prepared from the processed radix aconiti carmichaeli, the rhizoma zingiberis, the radix glycyrrhizae preparata, the poria cocos, the bighead atractylodes rhizome, theradix ginseng rubra, the white paeony root, the fructus corni, the fructus amomi, the herba ephedrae, the herba asari, the radix angelicae sinensis, the rhizoma cimicifugae and the radix puerariae, to the preparation of the medicine for regulating the intestinal flora balance.