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Rapid evaluation method of host caries risk based on Raman spectrum

PendingCN122084596ARealize early metabolic warningGuaranteed stabilityRaman scatteringBiotechnologyCell
The invention discloses a rapid evaluation method of host caries risk based on Raman spectrum, and relates to the technical field of microbiological detection. Aiming at the problem that in the prior art, it is difficult to rapidly and quantitatively reflect the early metabolic activity of the oral flora, the method introduces a stable isotope signal through heavy water labeling to obtain single-cell Raman spectrum information of the oral flora, and evaluates the metabolic activity of the flora on the basis of a characteristic parameter C-D ratio reflecting the metabolic state of microorganisms in a Raman spectrum, so that the early metabolic activity of the oral flora can be rapidly and quantitatively reflected. Therefore, the judgment of the caries risk of the host is realized. The method comprises the steps of sample collection, flora growth and metabolism law research under different concentration heavy water labeling, and metabolic activity detection under different environmental conditions. By adopting the heavy water concentration of 40%, on the premise that flora growth is not obviously influenced, rapid characterization of metabolic activity can be realized in a short time, the resolution ratio of single cells is high, and disturbance is small. The method provides a new tool for early warning of caries and oral flora metabolism research, and has a wide application prospect.
Owner:QINGDAO WOMEN & CHILDRENS HOSPITAL AFFILIATED TO QINGDAO UNIVERSITY

Intensive mobile beekeeping device

The utility model relates to an intensive mobile bee -keeping device, including box, modularization bee colony cabin and supporting cabin. The box top is equipped with lifting ring bolt interface convenient for transportation, and the bottom is equipped with honeybee import and export, and the left and right sides are equipped with supporting cabin and are isolated with bee colony cabin. Four bee colony cabins are equipped in the box, and three layers of nest base frame support frames are equipped in each cabin, and 900-1000 nest base frames can be hung. Intelligent management is realized through the temperature and humidity sensor, temperature control host and solar power supply system, and the support foot ensures the stability of placement. The device has the advantages of modularization design, convenient transportation, high space utilization, controllable environment and the like, and is suitable for large-scale, mobile bee -keeping operation.
Owner:ZUNYI VOCATIONAL & TECH COLLEGE