The invention discloses a rapid evaluation method of host caries risk based on Raman spectrum, and relates to the technical field of microbiological detection. Aiming at the problem that in the prior art, it is difficult to rapidly and quantitatively reflect the early
metabolic activity of the oral
flora, the method introduces a stable
isotope signal through
heavy water labeling to obtain single-
cell Raman spectrum information of the oral
flora, and evaluates the
metabolic activity of the
flora on the basis of a characteristic parameter C-D ratio reflecting the
metabolic state of microorganisms in a Raman spectrum, so that the early
metabolic activity of the oral flora can be rapidly and quantitatively reflected. Therefore, the judgment of the caries risk of the host is realized. The method comprises the steps of
sample collection, flora growth and
metabolism law research under different concentration
heavy water labeling, and metabolic
activity detection under different environmental conditions. By adopting the
heavy water concentration of 40%, on the premise that flora growth is not obviously influenced, rapid characterization of metabolic activity can be realized in a short time, the resolution ratio of single cells is high, and disturbance is small. The method provides a new tool for early warning of caries and oral flora
metabolism research, and has a wide application prospect.