The invention discloses an aluminum-scandium alloy target blank, and a preparation method and an application thereof, and belongs to the technical field of aluminum-scandium alloys. The aluminum-scandium alloy target blank prepared by combination of cold crucible levitation melting and variable-frequency electromagnetic stirring has an oxygen content of below 100 ppm and a total aluminum and scandium element content of 99.95 mass%, and has a high purity and a low oxygen content, so later high-quality film formation is facilitated; the volume percentage of a secondary precipitated phase in thealuminum-scandium alloy target blank is 5-25%, the average size is 20-45 [mu]m, and the blank has polygonal and nearly spherical shapes, a uniform microstructure and a significantly improved density,so micro-cracks and other internal defects in the ingot casting and deformation processing processes are effectively avoided, and the quality and yield of a target are significantly improved.