The invention relates to the technical field of medical instruments, and particularly discloses an automatic antibiotic bottle cap detection device, comprising a feeding mechanism, a vibration mechanism, a first conveying mechanism, a first detecting mechanism, a second detecting mechanism, a turning mechanism, a second conveying mechanism and a third detecting mechanism, wherein the feeding mechanism comprises a buffer plate, a vibration motor, a vibration plate, a contact rod and an inductor; the vibration mechanism comprises a first spiral conveying plate, a second spiral conveying plate, athird spiral conveying plate, a first steering track, a second steering track and a third steering track; the first detecting mechanism comprises a first light source and a first camera; the second detecting mechanism comprises a square fixed frame, a second light source, a fixed rod and a second camera; and the third detecting mechanism comprises a three camera and a third light source; the turning mechanism comprises a semi-closed sleeve, a torsion bar and a U-shaped buckle. The automatic antibiotic bottle cap detection device has the characteristics that the output can be adjusted, the positive and negative adjustments are unified, the transportation is not disturbed, the equipment cost is low, and the detection efficiency is high.