The invention relates to the technical field of automobile transportation, in particular to a multi-space cold chain transportation carriage based on different-temperature refrigeration. The carriage comprises a refrigerator, a carriage, two doors, a processor and a plurality of temperature sensors; the refrigerating machine comprises a compression outer unit and an air outlet inner unit, and a heat preservation protective cover is fixedly connected to one side outside the compartment; a plurality of delivery pipes are arranged on one side of the heat preservation protective cover, a plurality of air outlet holes are formed in one side of the compartment, the delivery pipes are fixed in the air outlet holes, a plurality of air return holes are formed in one side of the compartment, the delivery pipes are fixed in the air return holes, and automatic flow control devices are arranged on the inner sides of the delivery pipes. According to the invention, a separation door A, a separation door B, a connecting seat A, a connecting seat B, a door locking piece, the transfer pipe, a first sliding piece, a second sliding piece, a lifting piece, a precise motor and the temperature sensors are arranged. The carriage has the functions of multi-space transportation, quick connection, quick disassembly, automatic flow control, automatic constant temperature, automatic temperature regulation and the like.