34 results about "Surgical reattachment" patented technology

The invention discloses a tissue culture rapid propagation method for plukenetia volubilis L., also known as plukenetia volubilis, inca inchi, inca peanut and the like, is a perennial woody vine oil plant of the euphorbiaceae, and is native to the tropical rainforest of the Andes mountain area of South America. Vegetable oil squeezed from plukenetia volubilis L. seeds is regarded as the best vegetable oil in the world, and can be used for the aspects of food, health, pharmacy, cosmetics and the like. At present, the plukenetia volubilis L. seedlings are mainly cultivated through seed and cutting manner, and the problems of long period, high cost, low efficiency and the like exist, so that plukenetia volubilis L. in-vitro replantation plant is successfully obtained by taking the plukenetia volubilis L. section stem as an explant through processes of adventitious bud induction, multiplication, rootage, acclimatization, transplantation and the like, a plukenetia volubilis L. tissue culture rapid propagation technical system is established, and important significance is achieved for rapid propagation and large-area popularization of a good variety of the plukenetia volubilis L., as well as promotion of plukenetia volubilis L. industrial development.

The invention relates to an apparatus for film peeling and replantation. According to the invention, the apparatus for film peeling and replantation comprises a fixing plate (102) for fixing a sheet component where a film is attached, a peeling roller (104) which is arranged above the fixing plate (102) and to which one side of the film is attached, a peeling roller moving unit(106) which moves the peeling roller (104) where one side of the film is attached so as to peel the film off the sheet component, an adhesive roller (108) which attaches a film separated from the peeling roller and retains the other side attaching to the sheet component, and an adhesive roller conveying unit(112) which moves the adhesive roller (108) to attach the peeled film to the sheet component again.

Methods and apparatus for preserving detached tissues, especially digits and limbs, which are detached as a result of traumatic amputation. By using the methods and apparatus, detached tissues can be preserved for greater lengths of time and are ultimately in a better condition for replantation surgery.

The invention relates to a facility peach production method. The facility peach production method has the advantages that high-quality facility peaches can be produced by the aid of measures such as container root restriction culture, bagged slow release fertilizer application, early greenhouse uncovering in late fruit enlarging periods and artificial moderate drought two weeks before fruit maturing, and problems of poor coloring, sour and light fruit flavor and the like of conventional facility peaches can be effectively solved; the produced peaches are good in coloring, high in soluble solid and soluble sugar content, low in titratable acid content and strong in flavor as compared with the conventional facility cultivated peaches, and decrease of the average single fruit weight can be prevented; an optional novel technology for producing high-quality peaches which are supplied to markets at early stages is provided by the facility peach production method; problems of low greenhouse or facility greenhouse utilization efficiency, replantation barrier of lands in peach production facilities which are used for many years, and the like can be solved.

The invention relates to a method for constructing tissue engineered cartilage by using a PCL kernel-containing material. The method comprises the steps of a, making a PLC kernel; b, making a PLC kernel-containing PGA / PLA compound ear bracket; c, making a PGA / PLA compound ear bracket; d, culturing cartilage cells; e, constructing cartilage auricle bracket; and f, nude mouse subcutaneous replantation of in-vitro constructed auricle: respectively carrying out nude mouse subcutaneous implantation on the cartilage cells and an ear bracket compound after culturing in vitro for a week. According to the method, the tissue engineered cartilage can be constructed by using the PLC kernel-containing material, so that enough strength is provided to resist the tension caused by the subcutaneous implantation and then the original complicated and precise shape is maintained.

This invention discloses a method for obtaining turnip mosaic antiviral preserved szechuan pickle by means of Hc-Pro gene inversion, the method takes twice subcultured cotyledon inducted wound-healing tissue as inversion receptor, and co-culture the tissue with meloidogynosis peasant bacillus culture liquid with Hc-Pro gene, then continuously sieve the inversed wound-healing tissue on antibiotics added medium, last do differentiation and replantation. Compared with current technics, the beneficial effects of this invention are:Hc-Pro gene inversion is process on cell, and this can improve inversion efficiency and inheritance stability of the gene transferred plant, and can obtain Hc-Pro gene taking plant instead of chimera gene transferred plant.

A disclosed preparation method for self-skull used in replantation technology comprises that the self-skull is obtained for replantation by performing decontamination, pathogen inactivation, deproteinization, degreasing, decellularization, freeze drying, disinfection and the like on self skull taken out after an operation. The preparation method has the advantages that the storage time is long, the appearance of the taken-out self-skull is not changed after the self-skull is processed, no rejection phenomenon exists after implantation, and the like.

The invention relates to a transgenosis construct and an application thereof in preparing a space-time adjustable liver damage model and discloses a construct combination used for preparing the space-time adjustable liver damage model. The construct combination comprises a construct expressing space-time adjustable liver damage induction induced gene and a construct expressing Cre recombinant enzyme. The invention also discloses cells containing the construct and a method for preparing the space-time adjustable liver damage model. The obtained animal model can efficiently induce the liver damage at any time, and provides an effective way for the research of hepatic cell transplantation and liver replantation.

The present invention provides a soil remediation organic fertilizer for overcoming a watermelon replantation obstacle, a preparation method thereof and an application thereof. Agricultural straws, a straw-decomposing inoculant, an N fertilizer, a P fertilizer and a K fertilizer are subjected to mixed fermentation to prepare the soil remediation organic fertilizer. The straw-decomposing inoculant is prepared through subjecting four strains to cross antagonism and cultivation. The above four strains are composed of bacillus subtilis, Trichodermareesei, lactobacillus plantarum and Lactobacillus acidophilus. The bacillus subtilis is used for inhibiting and killing harmful bacteria and pathogenic bacteria in the soil. The Trichodermareesei is used for decomposing straw fibers and quickly composting straws. The lactobacillus plantarum is used for inhibiting and killing insects and worm eggs in the soil. The Lactobacillus acidophilus is used for removing the autotoxicity residues of watermelons. The effects of fungicides are realized based on the mutual coordination of the fungicides, agricultural straws, N, P and K fertilizers. Therefore, the accumulation of soil-borne pathogens, the soil nutrient imbalance and the plant autointoxication, which are inducement for replantation and successive cropping obstacles, can be avoided. Meanwhile, soil organic matters are supplemented, and the soil aggregate structure is optimized. Therefore, the soil remediation organic fertilizer has the advantages of water retention, drainage and ventilation, so that the plant root system is developed. Plants are enabled to grow healthily.

The invention provides a skull flap preservation device with an activity maintaining function. The skull flap preservation device with the activity maintaining function comprises a box. One side of the box is open. At least three layers of transverse partition plates are sequentially arranged in a cavity of the box from top to bottom. A plurality of month frames are arranged above each layer of transverse partition plate. At least three layers of pull-type lattice drawers are arranged in each month frame. Carrying sliding strips in contact with the corresponding pull-type lattice drawers are arranged on two opposite inner walls of each month frame. A plurality of vertical partition plates are arranged in each pull-type lattice drawer, and a lattice used for storing a skull flap is formed between every two adjacent vertical partition plates. A month marker is arranged on the front side wall of each month frame. A plurality of layers of markers are arranged on the front side wall of eachlayer of pull-type lattice drawers. By adoption of the skull flap preservation device with the activity maintaining function, the activity of skulls can be effectively maintained, the skulls are protected against contamination from the outside, accurate and rapid positioning is achieved, and errors are avoided; the influence on other skulls when one skull is stored or taken is reduced; and the infection rate after skull replantation is decreased, and the healing rate of patients is increased.

Provided is a fixed bracket for replantation of severed palms and fingers of a hand. The fixed bracket is formed by a bracket body and a binding belt, and the bracket body is the main part of the fixed bracket. The bracket body is a rectangular plate-type piece, and four edges of the bracket body are bent to be 90-degree flangings in the same direction. A plurality of rounding tooth-type notches are formed in the flanging of each edge and used for penetrating the binding belt binding the injured hand, and the number of the notches in the flangings is different. A U-shaped rabbet is further formed in one edge in the length direction of the bracket body and used as a hand placing space. The binding belt is a soft belt and is wound on the bracket body. The fixed bracket is used for fixing and supporting in the surgery process for severed palm and finger replantation of the hand.

The invention discloses a preparation method of a rapidly decellularized unifoliate liver biological scaffold. An ionic detergent sodium dodecyl sulfonate (SDS) with the problem that thrombus is easy to form after tissue transplantation and the problem of low replantation rate of decellularized tissues in a composite cellularization process is not used; a decellularized left outer-lobe liver biological scaffold is prepared by a weakly alkaline Triton solution perfusion method; due to the addition of NaOH in the Triton solution, the neutral Triton solution is in alkalescence; the cell lysis removal effect is enhanced; the time required for removing cells is shortened; and the optimal concentration and dosage of the NaOH and the Triton solution are adopted, so that the decellularization effect is the best.

Disclosed is a collagen material for promoting the survival of the skin with degloving injuries and avulsion injuries. The collagen material is composed of a collagen sponge and a nutrient solution. Specifically, bovine achilles tendons and pig achilles tendons which serve as raw materials are subjected to enzymatic hydrolysis and purification, collagen is prepared through fine extraction, the collagen sponge is obtained after crosslinking and freeze-drying treatment, and the nutrient solution is obtained. The nutrient solution is added while the collagen sponge is implanted in a wound. The collagen material has the advantages that the collagen material is implanted conveniently and easily applied to the surgical operation, the survival of the skin with the degloving injuries and the avulsion injuries is effectively promoted, the early recovery of the functions of the affected limbs is promoted, the necrosis rate of the skin with the degloving injuries or the avulsion injuries is reduced, the situation that multiple operations are caused by the occurrence of skin necrosis and complications of bone-tendon exposure is avoided, and the surgical complications caused by replantation surgery or flap surgery and the like are reduced to the greatest degree; the collagen material is suitable for hand and foot surgery and treatment of degloving injuries and avulsion injuries of the fourlimbs, and applicable to popularization and application.

Methods and apparatus for preserving detached tissues, especially digits and limbs, which are detached as a result of traumatic amputation. By using the methods and apparatus, detached tissues can be preserved for greater lengths of time and are ultimately in a better condition for replantation surgery.

The invention relates to an upper limb abdomen pedicled skin flap fixator, and belongs to medical instruments. The upper limb abdomen pedicled skin flap fixator is used for the treatment of replantation operation of severed limbs, and consists of a base, an arm support plate of which one end is hinged on the base, a bundling belt arranged on the arm support plate and a mechanical lifting mechanism which is connected between the base and the arm support plate, so that the arm support plate is lifted and drops relative to the base. The upper limb abdomen pedicled skin flap fixator has the advantages that an arm position fixing device is provided, the unconscious movement of arms is avoided, the phenomena of influence on blood supply of skin flaps and vein backflow which are caused by the folding and torsion of skin flap pedicles due to the movement of the arms are eliminated, and the union of wounds is facilitated. The upper limb abdomen pedicled skin flap fixator has an adjustable height and angle and a stable and firm structure, is high in adaptability and generality, and can be used safely and reliably.

The invention relates to the soybean plantation, especially the replantation; the technical project of the invention to replant soybean is as following: cultivate the soybean seedlings for precocity, winter resistance, resisting drought and childbearing period about 84 days, and move them into farmland to ramify from rap roots and lateral roots and sizing, plant the second ones in the middle , and they can go on growing after the harvest of the first season ones. In Heilongjiang district, after the first season soybean, plant the second season soybean in the middle of the first one, and they can go on growing after the harvest of the first season ones. In Liaoning Province, if planted after the first ones have been harvested or before the mature, the second season soybean will have the fatness as follows: restisting high temperature, resisting drought, resisting ventitation, and prematurity and developed caulis. It can survive well in barren and ramify prosperously. This soybean grows flourish and mature in short time. The procreating period is 84 days, and all the active accumulated temperature is 1700 centigrade degrees. The method above can be used in south areas to harvest 3 times.

The invention relates to a culture apparatus for an amputative limb or a severed finger, which comprises a container, wherein perfusate is filled into the container; a liquid output end of the perfusate container is communicated to a blood pump for supplying perfusate power through a pipe; a liquid output end of the blood pump is communicated with an oxygen-laden artificial lung for supplying blood and gas exchange through a pipe; a liquid output end of the artificial lung is communicated with an arterial blood vessel of the amputative lumb or the severed finger through a pipe; and the amputative limb or the severed finger is placed into an airtight constant temperature monitor. The culture apparatus for the amputative limb or the severed finger can reduce the tissue metabolic rate of the amputative limb or the severed finger under the isolated state, recovers and maintains tissue survival of the amputative limb to the maximum degree, eliminates necrotic tissue and toxin, and converts the replantation time limit of the amputative limb into a parameter capable of being manually controlled and effectively prolonged.

The invention discloses a thumb replantation board. The thumb replantation board comprises a square box and fixing rods, wherein the fixing rods are distributed on two long lateral sides and one short side of the square box; an upper arc-shaped slope groove is formed in the upper surface corresponding to the other short side with the fixed rod; the upper arc-shaped slope groove matches with an arc shape when the root of a palm is horizontally fixed; a lower arc-shaped slope groove is formed in the lower surface of the square box which is corresponding to the position of the upper arc-shaped slope groove. By adopting the above manner, 2-5 fingers can be well fixed; a fixing rubber band is easy and simple to buckle; after fixation, doctors can well perform an operation, thus survival rate of a replanted thumb is improved; the thumb replantation board is easy to clean, simple in structure, convenient to use and applicable to large-scale production.

The invention relates to a human amputated limb protection box based on non-contact sensing control perfusion technology, belongs to the field of medical auxiliary equipment, and provides a protectionbox which has reasonable structural design, can replace residual waste caused by ischemic injury, can be added with appropriate drugs for targeted treatment at the same time, can maintain the activity of isolated amputated limbs, further improves the replantation success rate and survival rate, and reduces the occurrence of limb disability. A condenser tube is arranged at the bottom of the protection box, a placing plate is arranged at the upper end of the condenser tube, a mounting plate is arranged at the middle end of the protection box, water is poured into the lower end of the mounting plate, a storage box, an oxygen cylinder and a medical peristaltic pump are arranged at the upper end of the mounting plate, a perfusion liquid storage box and a perfusion liquid buffer pool are arranged at the upper end of the placing plate, and then all devices are connected with each other through pipelines to form a perfusion pipeline, and nutrients required for maintaining the activity of theamputated limbs are supplied through the circulation flow of a perfusion liquid.

The invention relates to the field of three-dimensional printing preparation of nucleus pulposus replantation bodies, in particular to a method of preparing a nucleus pulposus replantation body in combination with a three-dimensional printing technology. According to the method of preparing the nucleus pulposus replantation body in combination with the three-dimensional printing technology, a polycaprolactone elastomer support is prepared by three-dimensional printing to serve as a mechanical support material of a nucleus pulposus implant; nucleus pulposus waste is treated to form a compositesupport with a polycaprolactone elastomer; and the support is good in mechanical property and biocompatibility, and contains a nucleus pulposus extracellular matrix and nucleus pulposus cells. The method of preparing the nucleus pulposus replantation body through the three-dimensional printing to treating degeneration of the lumbar intervertebral disc can obtain a series of polycaprolactone elastomer support products with different specifications via printing; the products are dry solids, and are easy to store and long in storage life; and at the same time, the nucleus pulposus replantation body can be replanted after immediate treatment intraoperatively by a replantation method after compositing of the nucleus pulposus waste and the polycaprolactone elastomer support.

The invention discloses a tissue culture rapid propagation method for plukenetia volubilis L., also known as plukenetia volubilis, inca inchi, inca peanut and the like, is a perennial woody vine oil plant of the euphorbiaceae, and is native to the tropical rainforest of the Andes mountain area of South America. Vegetable oil squeezed from plukenetia volubilis L. seeds is regarded as the best vegetable oil in the world, and can be used for the aspects of food, health, pharmacy, cosmetics and the like. At present, the plukenetia volubilis L. seedlings are mainly cultivated through seed and cutting manner, and the problems of long period, high cost, low efficiency and the like exist, so that plukenetia volubilis L. in-vitro replantation plant is successfully obtained by taking the plukenetia volubilis L. section stem as an explant through processes of adventitious bud induction, multiplication, rootage, acclimatization, transplantation and the like, a plukenetia volubilis L. tissue culture rapid propagation technical system is established, and important significance is achieved for rapid propagation and large-area popularization of a good variety of the plukenetia volubilis L., as well as promotion of plukenetia volubilis L. industrial development.

The invention discloses a formula and a boiling method for a traditional Chinese medicine having repairing efficacy after replantation of a severed finger. In the formula, draconis resin promotes blood circulation and relieves pain, removes stasis to stop bleeding, and regenerates tissue to heal wond; radix notoginseng dissipates blood stasis to stop bleeding, and dispersing swelling and settlingpain; doubleteeth pubescent angelica roots dispells wind and eliminates dampness, and relieves pain of arthralgia aggravated by cold; safflowers and Sichuan lovage rhizome have the effect of promotingblood circulation to remove blood stasis; frankincense and myrrh are together utilized to promote blood circulation, promote qi and relieve pain, and disperse swelling and engender flesh; incised notopterygium rhizome and roots disperse exterior cold, expel wind-damp, are beneficial to arthrosis, and relieve pain; dahurian angelica roots dispel wind, dry dampness, disperse swelling, and relieve pain; Chinese angelica has the effect of tonifying qi and replenishing blood; fortune's drynaria rhizome and himalayan teasel roots have the effect of reunion of fractured tendon and bone; traditionalChinese medicines such as puerarin and rose-boots having the effects of promoting blood circulation to remove blood stasis, benefiting qi for activating blood circulation, promoting qi circulation andremoving obstruction in the collateral(s), and the like may have an effect of promoting blood vessel regeneration; and aloe has antibacterial and anti-inflammatory effects, treats tissue damage, protects skin, and can stimulate growth. Through the effects of setting a bone and replenishing the bone, promoting the blood vessel regeneration, stopping the blood and engendering the flesh, promoting the blood circulation and being beneficial to the arthrosis, the traditional Chinese medicine has a remarkable curative effect on repair of replantation of severed fingers.

The invention discloses a toe replantation plate which comprises a retaining block and two fixed blocks fixed at the two ends of the retaining block. The retaining block and the fixed blocks are each shaped like a rectangle, the retaining block is flush with the lower surfaces of the fixed blocks, and the height of the retaining block is a half to two thirds of that of the fixed blocks; the fixed blocks are arranged symmetrically, and the retaining block is located at the ends of the fixed blocks and is perpendicular to the fixed blocks. In this way, 90-degree position fixation of the ankle can be effectively maintained, the foot with problems is limited to fall left and right and move back and forth, and the broken toe can conduct turning operation conveniently. Meanwhile, a platform can be provided for containing the forearm and elbow of a surgeon.

The invention discloses oral administration medicine tea for severed finger, toe and limb microsurgery replantation recovery. The tea comprises the following components by weight: 15g of acanthopanax lasiogyne harms, 10g of crude cortex acanthopanacis, 15g of eleutherococcus leucorrhizus, 10g of streptocarpus clarkeanus, 5g of panax japonicus, 5g of moutan bark, 10g of chinese redbud bark, 5g of girald daphne bark, 5g of pterospermum grande, 15g of viburnum odoratissimum root, 15g of teasel root, 15g of uncaria tonkinensis, 10g of ampelocissus artemisiaefolia, 10g of liquorice and 15g of coix seed. The oral administration medicine tea for severed finger, toe and limb microsurgery replantation recovery is remarkable in medicine effect, can effectively relieve sequelae of severed finger, toe and limb microsurgery replantation, can improve the survival rate of replanted severed fingers and limbs, can promote recovery of severed fingers and limbs, and can further reduce the wound infection rate in severed finger, toe and limb microsurgery replantation.

The invention provides an antagonistic bacterium against Fusarium moniliforme heterophylla and its application. The antagonistic bacterium has been identified as Pseudomonas LK‑313, and its preservation number is CGMCC NO.12121. The antagonistic bacteria provided by the present invention are screened and obtained from the rhizosphere soil of Radix Pseudostellariae by Pseudomonas selective medium, plate confrontation culture shows that the antagonistic bacteria can effectively inhibit the growth of mycelia of pathogenic bacteria such as Fusarium moniliforme of Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix or Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix by the antagonistic bacteria of the present invention. It has good colonization ability and no pathogenicity to other crops. Simultaneously, the present invention provides the optimum culture medium for the growth of the antagonistic bacteria. The optimal formula is 1 / 4 LB+1 / 20 MS (without iron salt)+0.1wt.% brown sugar, and the fermented bacteria liquid has obvious antagonistic effect. The invention provides antagonistic bacteria strains and bacterial agents for overcoming or alleviating the replanting diseases of the medicinal plant Pseudostellariae Pseudostellariae, which has good biocontrol effect, no pollution, safe ecology, and broad development and application prospects.

The invention relates to a planting method for corn replantation. According to the technical scheme of the planting method for corn replantation, corn seedlings which mature early and are resistant to cold and drought are cultivated at first and transplanted to a field to be subjected to lateral root and main root branching and grain filling till mature, wherein the growth period of the corn seedlings is about 84 days, and the active accumulated temperature of the whole growth period is 1700 DEG C; the corn seedlings in the second season are replanted in the middles of the corn seedlings in the first season and can also be planted on the peripheries of roots of the corn seedlings in the first season, or are planted before the corn seedlings in the first season mature; and the corn seedlings in the second season have the characteristics of being resistant to high temperature, draught and air permeability, early mature, well developed in stem, high in barren tolerance and branchiness and quick in vegetative body growth and maturity, the growth period is about 84 days, and the active accumulated temperature of the whole growth period is 1700 DEG C. By means of the planting method for corn replantation, three seasons of corn production can be achieved in the south.

The invention relates to a transgenosis construct and an application thereof in preparing a space-time adjustable liver damage model and discloses a construct combination used for preparing the space-time adjustable liver damage model. The construct combination comprises a construct expressing space-time adjustable liver damage induction induced gene and a construct expressing Cre recombinant enzyme. The invention also discloses cells containing the construct and a method for preparing the space-time adjustable liver damage model. The obtained animal model can efficiently induce the liver damage at any time, and provides an effective way for the research of hepatic cell transplantation and liver replantation.

The invention provides an early non-destructive identification method for nitrogen absorption capacity of a tea tree, which belongs to the technical field of tea tree cultivation. The method comprisesthe steps of 1) tea tree cutting branch selection and cutting and root selection and pretreatment; 2) non-invasive micro-test root system fixing; 3) test solution selection; 4) non-invasive micro-test system correction; 5) root system surface NH4+ and NO3-ion flow rate determination; 6) tea tree nitrogen absorption capacity evaluation; and 7) cutting seedling replantation. According to the earlynon-destructive identification method for the nitrogen absorption capacity of the tea tree, the tea tree resource is subjected to sand culture cutting for a period of time, and then the tea seedling is rooted out; non-invasive micro-test of the nitrate and ammonium ion flow rate of the tea seedling root is carried out; and the nitrogen absorption capacity of the tea tree resource is evaluated.

The invention provides a spinous process vertebral plate replantation internal fixing device. The device is suitable for bone replantation fixation of a spinous process (11) and / or a vertebral plate (12) after intravertebral surgery. The device comprises one or more fixing units, wherein each fixing unit comprises a fixing plate (2), a fixing piece (21) and a bone grafting bin (22); the fixing piece (21) is arranged on the fixing plate (2), and the fixing plate (2) is fixedly connected to the spinous process (11) and / or the vertebral plate (12); and the bone grafting bin (22) is connected to the fixing plate (2). The device has the advantages of simple structure, light weight, small volume and high reliability; through the arrangement of at least three fixing pieces, the fixing effect is effectively improved, and the risk of lateral dislocation of the vertebral plate is reduced; the fixing effect can be further improved by arranging a pedicle screw and / or a facet joint screw; and through the specially-designed reticular bone grafting bin structure, the tissue growth of the operation area can be promoted, and the compression can be reduced.