The invention discloses a tissue culture rapid propagation method for plukenetia volubilis L., also known as plukenetia volubilis, inca inchi, inca peanut and the like, is a perennial woody vine oil plant of the euphorbiaceae, and is native to the tropical rainforest of the Andes mountain area of South America. Vegetable oil squeezed from plukenetia volubilis L. seeds is regarded as the best vegetable oil in the world, and can be used for the aspects of food, health, pharmacy, cosmetics and the like. At present, the plukenetia volubilis L. seedlings are mainly cultivated through seed and cutting manner, and the problems of long period, high cost, low efficiency and the like exist, so that plukenetia volubilis L. in-vitro replantation plant is successfully obtained by taking the plukenetia volubilis L. section stem as an explant through processes of adventitious bud induction, multiplication, rootage, acclimatization, transplantation and the like, a plukenetia volubilis L. tissue culture rapid propagation technical system is established, and important significance is achieved for rapid propagation and large-area popularization of a good variety of the plukenetia volubilis L., as well as promotion of plukenetia volubilis L. industrial development.