The invention discloses a washable antibacterial super-hydrophobic coating and a preparation method and application thereof.According to the method, hydrophobic interaction is utilized, after hydrophobic nano-particles form a compact hydrophobic particle film on the water surface, a drainage sedimentation technology is changed into a printing technology, namely, a base plate with a double-face bonding layer is vertically placed into water, then the base plate is rotated to the horizontal angle to be slowly lifted, and the base plate is subjected to water washing; and the particle film is attached to the double-sided bonding layer, heating is carried out to enhance the bonding force of the particle film and the double-sided bonding layer, and the super-hydrophobic coating is obtained. The preparation method takes water as a medium, does not need to use an organic solvent, and has the characteristics of simple process and environmental protection; the printing process can greatly simplify the process flow, reduce the production cost and is suitable for large-area preparation. The prepared super-hydrophobic coating has the advantages of good antibacterial performance, good super-hydrophobic performance, good underwater stability, washability, good flexibility and the like, has high practical value and good application prospects, and can be applied to the fields of medical treatment and public health, food industry, antifouling, environmental protection and the like.