The invention discloses a zirconia ceramic fingerprint identification sheet and a preparing and application method thereof. The zirconia ceramic fingerprint identification sheet is prepared from a main material and auxiliary materials. The main material is zirconia powder, and the auxiliary materials are prepared from a bonding agent, a plasticizer, a dispersing agent and a solvent. The preparing method includes the following steps that the main zirconia powder material, the bonding agent, the plasticizer, the dispersing agent and the solvent are dispersed to be mixed to be even, and slurry capable of carrying out tape casting is obtained; the slurry is subjected to vacuum bubble removing, and a film forming belt is formed in a tape-casting mode; the tape-casting belt is dried to be crop, the product is put into a mold and subjected to punch forming, a punching blank with the required shape is prepared, constant temperature and isostatic pressing are carried out, and a raw blank is obtained; the raw blank is degreased and subjected to glue discharging, and then the product is sintered into ceramic sheets; the ceramic sheets are appearance processing and polished, and then the zirconia ceramic fingerprint identification sheet is obtained. The zirconia ceramic sheet prepared with the method is high in strength, toughness and size accuracy. The tape-casting processing mode is adopted, the following processing amount is small, the preparing production efficiency is high, and large-scale production can be met.