The invention relates to a multi-functional navigation puncture treating template. The multi-functional navigation puncture treating template comprises a template body, puncture main needle holes, puncture subsidiary needle holes, marking lines and lead numbers, puncture needle holes with different sizes distributed in rows and in columns are evenly arranged on the template body, the lead numbers are placed at the positions, corresponding to left side needle holes, of row marking lines as row marks, and lead threads are placed at the positions, corresponding to the upper portion of the needle holes, of column marking lines as column marking lines. The multi-functional navigation puncture treating template can fast determine the positions of the needle holes, precisely guide the puncturing direction of a puncture needle, the puncture needle is precisely inserted, the operation time is shortened, the operation risk is lowered, and the operation difficulty is reduced. Different puncture holes can simultaneously combines a puncture biopsy with various kinds of minimally invasive treatment, the puncture biopsy is conducted at the same time of the treatment, and the pain of a patient is lowered; various kinds of the minimally invasive treatment can also be combined, all own advantages are given play to, the disadvantages of various kinds of the minimally invasive treatment are avoided, the maximum tumor treating effect is achieved, and the combined operation not only relieves the burden on the patient, but also improves the curative effect of tumor treatment.