41 results about "Genus Shigella" patented technology

With the goal of creating a combination vaccine against Shigella and other diarrheal pathogens we have constructed a prototype vaccine strain of Shigella flexneri 2a (SC608) that can serve as a vector for the expression and delivery of heterologous antigens to the mucosal immune system. SC608 is an asd derivative of SC602, a well-characterized vaccine strain, which has recently undergone several phase 1 and 2 trials for safety and immunogenicity. Using non-antibiotic asd-based plasmids, we have created novel constructs for the expression of antigens from enterotoxigenic E. coli (ETEC), including CFA / I (CfaB and CfaE) and the B-subunit from heat-labile enterotoxin (LTB) in Shigella vaccine strain SC608. Heterologous protein expression levels and cellular localization are critical to immune recognition and have been verified by immunoblot analysis. Following intranasal immunization (SC608(CFAI) and SC608(CFAI / LTB) of guinea pigs, serum IgG and IgA immune responses to both the Shigella LPS and ETEC antigens can be detected by ELISA. In addition, ELISPOT analysis for ASCs from cervical lymph nodes and spleen showed similar responses. All vaccine strains conferred high levels of protection against challenge with wild-type S. flexneri 2a using the Sereny test. Furthermore, serum from guinea pigs immunized with SC608 expressing CfaB and LTB contained antibodies capable of neutralizing the cytological affects of heat-labile toxin (HLT) on Chinese Hamster Ovary (CHO) cells. These initial experiments demonstrate the validity of a multivalent invasive Shigella strain that can serve as a vector for the delivery of pathogen-derived antigens.

The invention relates to a detection reagent for a Shigella flexneri plasmid-carried gene Ipt-O and an application thereof, and in particular relates to an application of the detection reagent for a Shigella flexneri plasmid-carried gene Ipt-O in preparation of a Shigella flexneri serotype detection agent. The detection agent is used for detecting the Shigella flexneri serotype or detecting the differential diagnosis sample containing Shigella flexneri. The invention also relates to a reagent for Shigella flexneri MASF IV-1 phenotype detection, which is composed of a primer pair of the Shigella flexneri plasmid-carried gene Ipt-O as shown in the SEQ ID No.2 and SEQ ID No.3, and further relates to a method for detecting the Shigella flexneri MASF IV-1 phenotype by using the primer pair, and a method for distinguishing Shigella flexneri Xv serotype and X serotype, 4a serotype and 4av serotype as well as Y serotype and Yv serotype.

The present invention provides a LAMP primer and a detection method for detecting Shigella flexneri 2a and Xv serotypes. The present invention designs LAMP primers according to the type-specific antigenic determinants and group-specific antigenic determinants of the main serotypes 2a and Xv of Shigella flexneri, wherein the 2a serotype is (gtrII and wzx genes are positive, gtrX and opt genes are negative) ), Xv serotype (gtrX, opt and wzx genes positive, gtrII gene negative). The method does not require expensive equipment and instruments, and can rapidly, accurately and specifically detect Shigella flexneri 2a and Xv serotypes.

The invention discloses a making method of short peptide to inhibit shigellosis with amino acid as list 1, which is characterized by the following: adding chemical group, amino acid, peptide, protein, PEG, marked material to N and C ends to do kinds of chemical or biological decoration; optimizing the stability or other applying property and using scale; fitting for treat shigellosis, enterorrhagia escherichia coli O157 and cholera vibrio.

A method, comprising the steps of providing a sample containing contaminating nucleoside diphosphates and / or nucleoside triphosphates, such as ATP and / or ATP analogues including deoxyribonucleoside triphosphates;reducing the amount of the contaminating nucleoside diphosphates and / or nucleoside triphosphates in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneriapyrase; andperforming an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates and / or nucleoside triphosphates had they not been reduced in the reduction step.