The invention discloses an automatic demoulding vulcanization molding die and a manufacturing method for a wire harness sealing ring with a sugarcoated haws structure, and belongs to the technical field of rubber product production. The vulcanization molding die comprises a lower die, a middle die, an upper die, a top die and a die core, wherein the lower die, the middle die, the upper die and thetop die are sequentially arranged from bottom to top, the die core is arranged on the lower die, the lower side of the upper die is provided with a die cavity, the upper side of the upper die is provided with an injection cavity, the die cavity communicates with the injection cavity through an injection hole, and the lower side of the top die is provided with an injection plug; after the lower die, the middle die, the upper die and the top die are matched, the die core can penetrate through the middle die and enter the die cavity, the top of the die core is inserted into the injection hole, and the injection plug is embedded into the injection cavity. According to the method, the wire harness sealing ring with the sugarcoated haws structure can be manufactured by adopting the vulcanization molding die. The wire harness sealing ring can be automatically demoulded after the wire harness sealing ring is vulcanized, a threading hole cannot be broken, the die core cannot be damaged, the product qualification rate is high, and the production efficiency is high.