The invention provides a disinfecting traditional Chinese medicine composition, a traditional Chinese medicine preparation, a preparation method of the traditional Chinese medicine preparation, and application of the disinfecting traditional Chinese medicine composition and the traditional Chinese medicine preparation, belonging to the technical field of traditional Chinese medicine compositions.The traditional Chinese medicine composition comprises the following raw materials: divaricate saposhnikovia roots, honeysuckle flowers, radix paeoniae rubra, schisandra chinensis, folium artemisiae argyi, flos caryophylli, rhizoma curcumae longae, angelica sinensis, folium isatidis, herba menthae, cortex cinnamomi, herba schizonepetae, platycodon grandiflorum, amomum kravanh, radix adenophorae and liquorice roots. The traditional Chinese medicine composition disclosed by the invention has multiple effects of killing bacteria, fungi and viruses, and also has the effects of regulating the bodyfunctions and metabolic functions of a human being. Moreover, the raw materials of the traditional Chinese medicine composition disclosed by the invention are natural, safe, non-toxic, residue-free and resistance-free, and drug resistance is not easy to generate. Under the condition that people exist, safe and effective disinfection can be achieved, and no harm is caused to the human body and theenvironment. The traditional Chinese medicine composition is fragrant in smell and has the effect of refreshing air; and aromatic plants such as honeysuckle, artemisia, mint and cinnamon contained inthe raw materials can kill microorganisms in the air and have the effect of refreshing the air.