The invention discloses a bio-based degradable film blowing master batch and a production process thereof. The bio-based degradable film blowing master batch comprises the following components: biological refined starch, polyethylene resin, a compatilizer, a coupling agent, an antioxidant and a lubricant. The process comprises the following steps: a, obtaining biological refined starch; b, removing impurities from the biological refined starch obtained by refining; c, heating and dehydrating the biological refined starch; d, mixing the polyethylene resin, the biological refined starch, the lubricant, the compatilizer, the coupling agent and the antioxidant; e, feeding the materials into an automatic feeding device; f, sealing the automatic feeding device and the double-screw extruder; g, extruding to form master batches; and h, the material conveying pipe, the first hose and the second hose are cleaned. The obtained master batch can be degraded in the natural environment, so that the environmental pollution is reduced. Meanwhile, the material conveying stability is good, the prepared materials can be uniformly and continuously fed into the double-screw extruder through the automatic feeding device, and the production efficiency of the master batch is improved.