The invention discloses an air conditioning testing and regulating device which comprises an air conditioner to be debugged, an electronic expansion valve, a refrigerant increasing and decreasing device, a value control unit and a central control module, wherein the air conditioner to be debugged comprises a compressor, a condenser and an evaporator which are connected through a refrigerant pipeline, the electronic expansion valve is detachably installed on a pipeline between the condenser and the evaporator, the refrigerant increasing and decreasing device is detachably connected with the refrigerant pipeline, the value control unit is used for controlling the action of the electronic expansion valve, and the central control module is used for controlling the value control unit. The invention also discloses an air conditioning testing and regulating method. The size of a path of the electronic expansion valve and the volume of the refrigerant are regulated by the central control module. With the adoption of the air conditioning testing and regulating device and method, the debugging process of an air conditioning is greatly simplified, the debugging time of the air conditioning is saved, the production efficiency of the air conditioning is enhanced, and the production cost of the air conditioning is lowered.