The invention provides a preparation method of a rectangular separation net for deep and far sea culture, which comprises the following steps: preparing a blended antifouling composite material, preparing a light blue monofilament bundle, preparing a rectangular separation net, and mixing polypropylene granules, a polyhexamethylene guanidine hydrochloride grafted modified polypropylene antifouling material, nano copper powder, pyrithione copper powder, tetra [beta-(3, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4- 2, 5-di-tert-butyl-4-hydroxyphenyl) propionic acid] pentaerythritol ester, tri (1, 2, 2, 6, 6-pentamethylpiperidinyl) phosphite, phthalocyanine blue pigment, titanium dioxide and turpentine are mixed and uniformly stirred, extrusion granulation is performed 2-3 times through a double-screw extruder to obtain a blended antifouling composite material, light blue monofilament tows are obtained after wire drawing and rolling, and the light blue monofilament tows are used as raw materials. And obtaining the rectangular separation net for deep and far sea culture by using a special rectangular separation net preparation method.