32results about How to "Improve light and effect" patented technology

The invention discloses a brassica napus BnPABP8 promoter and a preparation method and use thereof. The preparation method comprises the following: A, a step of primer design, which is to design specific walking primers according to known sequences; B, a step of brassica napus promoter preparation, which is to extract brassica napus DNA by using a sodium dodecyl sulfonate (SDS) lysis method, perform genome walking according to genome walking kits, digest 5 micro liters of DNA by using DraI enzyme, detect the purity of the DNA, perform enzyme digestion of the DNA by using the incision enzymes provided by four kits, and purify the product of the enzyme digestion; C, a step of purification, which is to add ethanol into enzyme digestion solution, precipitate, centrifuge, dry in the air, dissolve in sterile water and add specific connectors after purification is accomplished, wherein GSP1 and AP1 are used as primers in primary polymerase chain reaction (PCR) reaction; and D, a step, which is to perform amplification by using the diluted solution of the product of the primary PCR reaction as a template and GSP2 and AP2 as primers in a secondary PCR reaction, recover purified product of the secondary PCR product, connect with a T vector, and obtain the PBnPABP8. The invention also discloses the use of the PBnPABP8 in roots, stems, leaves, buds and anthers of plants. When the promoter is used, the safety of the edible brassica napus oil can be improved, and the disease resistance, stress resistance and lodging resistance in crops can be improved by transgenosis.

This invention discloses a method for producing compound boron fertilizer, which is white or light green crystal, and mainly composed of: B 1.0-1.2 wt.%, Zn 0.2-0.5 wt.%, Fe 0.6-1.0 wt.%, and Mg more than 7.0 wt.%. The method comprises: preparing concentrated solution, preparing Zn-Fe mixed solution, preparing semi-finished product, and preparing the final product. The compound boron fertilizer contains Fe and Zn that are necessary for the growth of cotton and rape. Thus, the compound boron fertilizer can compensate the defects of B-Mg fertilizer, promote the development of cotton and rape root systems, improve the ability to absorb nutrients, stimulate the development of gynoecia germens, accelerate the growth, promote photosynthesis, shorten the growth period, advance the mature time, and prevent deficiency symptom. The compound boron fertilizer has increased yield (by 15-25%), simple process, easy application, and no pollution.

The invention discloses a wheat foliar fertilizer. The wheat foliar fertilizer is characterized by comprising the following chemical fertilizer raw materials in parts by weight: 25-45 parts of carbamide (containing 46% of N), 15-35 parts of calcium superphosphate (containing more than 15% of P2O), 20-45 parts of potassium sulphate (containing 50% of K2O), 0.5-3 parts of magnesium sulphate, 0.5-1 part of ferrous sulfate, 1-3 parts of zinc sulfate, 20-30 parts of ammonium sulfate, 0.5-1 part of manganese sulfate, 0.2-1.5 parts of ammonium molybdate, 0.1-1 part of borax (containing more than 11% of B), 0.5-3 parts of citric acid, 0.2-0.6 part of a complexone, 0.1-0.5 part of a wetting agent, and 0.1-0.3 part of oxydol. The wheat foliar fertilizer has the advantages that zero contamination, lodging resistance, frost resistance, and pest and disease resistance are achieved, the cost is low, the effect is good, and crop yield and product quality are improved.

The invention discloses a method for preparing a promoter (PBnPABP2) of Brassica napus BnPABP2 and the application thereof. The operation of cloning a PBnPABP2 sequence by using a genome walking method comprises the following steps: extracting genome DNA by using a SDS cracking process; in different systems, carrying out restriction endonuclease reaction on the extracted DNA respectively by using endonucleases DraI, EcoRV, PvuII and StuI; after the obtained DNA is connected with a linker fragment, carrying out first-round PCR amplification by taking the linker fragment as a template; and carrying out second-round PCR amplification by taking a50-time diluent of the obtained first-round PCR product as a template, thereby obtaining the PBnPABP2. The PBnPABP2 is applied in the roots, stems, leaves, flower buds, anthers and peels of plants. The promoter has the function of driving the expression of exogenous genes, and the expression sites thereof are respectively in the roots, stems, leaves, flower buds, anthers and peels of plants; and the promoter has application potentials in the aspect of safety of edible oil of Brassica napus and the aspects of improving the disease resistance, stress resistance and lodging resistance of crops, improving the quality of crops, artificially creating a male sterile line and a restoring line, enriching germ plasm resources, and the like through transgenosis.

The invention relates to an agriculture agent, in particular to a mixed chemical for controlling cercospora black spot of peanut and a control method of cercospora black spot of peanut. The chemical for controlling the cercospora black spot of peanut of the invention comprises diniconazole, carbendazol and palmityl alcohol which are mixed evenly in proportion by weight of 1:1:1-2. Proven by testing, the combination bactericide of the invention has stable chemical property and obvious synergism, and presents obvious synergism and complementary effects on the controlled object.

The invention relates to three-microbialactivity multi-enzyme plant nutrient solution and a production process thereof, belonging to the field of fertilizers. The invention is characterized in that microorganism conversion solution, multielement organic nutrient solution and microfauna enzyme nutrient solution are compounded into the 'three-microbialactivity multi-enzyme plant nutrient solution'. The microorganism conversion solution is compounded from photosynthetic bacteria, bacillus and lactic acid bacteria; the multielement organic nutrient solution is compounded from five main life nutrient elements, namely selenium, germanium, chromium, zinc and calcium; and the microfauna enzyme nutrient solution is composed of maggot wormcast nutrient solution and is catalyzed by a '863 nano catalyst'. The comprehensive effects of promoting root growth, strengthening seedlings, resisting stress, preventing disease, resisting lodging, promoting precocity, realizing no residue, preventing pollution, realizing high yield and improving quality can be achieved. On the basis of the average yield peak of the traditional fertilizer or compound fertilizer which is applied sufficiently, the three-microbialactivity multi-enzyme plant nutrient solution can further improve the yield, particularly improves the yield of grain by 15-20%, the yield of vegetable by 40-80%, and the yield of fruit by 20-40%, thus the nutrient solution provided by the invention can be widely applied to planting of crops or plants.

The invention discloses a functional liquid fertilizer for an orchard in a salt-alkaline land and a preparation method thereof. The functional liquid fertilizer for the orchard in the salt-alkaline land is prepared from nutritional matters and biological active matters, wherein the nutritional matters comprise nitrogen, phosphor, potassium, boron and zinc; the biological active matters comprise glutamic acid, methionine, phenylalanine, levulinic acid, genistein and chitosan oligosaccharide. The functional liquid fertilizer has the advantages that the biochemical theory of fruit growth and development is used as the instruction concept; on the basis of mineral nutrients required by the fruit development, a biological stimulation factor for promoting forming of anthocyanin, a primer elicitorfor synthesizing of the anthocyanin and the like are added, so that the synthesizing of anthocyanin is obviously promoted, the fruit flavor is improved, the red fruit rate is obviously increased, andthe safety and high-efficiency in application are realized; the convenience in use is realized, the working period is shortened, the easiness in phytotoxicity is avoided, any toxic and side effect tohuman bodies and environments is avoided, and the like.

The invention relates to the technical field of fertilizers, and particularly discloses a medium-trace-element organic biological fertilizer and a preparation method thereof. The medium-trace-elementorganic biological fertilizer is prepared from the following components in parts by mass: 45-50 parts of mushroom bran, 15-20 parts of attapulgite, 20-32 parts of medium-trace-element fertilizer body,8-10 parts of biological bacterial fertilizer body, 3-5 parts of lignin peroxidase and 3-5 parts of cellulase, wherein the mushroom bran is a mixture of agrocybe cylindracea mushroom bran, lentinus edodes mushroom bran and oyster mushroom bran with the mass ratio of (3-5):(2-4):(1-3). The medium-trace-element organic biological fertilizer has the advantages that the soil nutrition structure is improved, the effective utilization of soil nutrients is improved, the medium trace elements which are indispensable in plant growth are provided, the photosynthesis of plants is enhanced, the root growth is promoted, and the growth and development of the plants are promoted. The preparation method has the advantages of being simple in process, convenient to operate, safe, environmentally friendly and easy to implement.

The invention discloses an organic fertilizer prepared by aerobic fermentation of microorganisms. A preparation method comprises steps: collecting fresh crop straws, and further crushing the collectedcrop straws into a crop straw suspension; adding peracetic acid into the crop straw suspension, and fully stirring; and carrying out aerobic fermentation by using enzyme microorganisms, adding functional zymophyte, and carrying out facultative fermentation for 2-5 days. The method can fully realize cyclic utilization of data, use microorganisms for aerobic fermentation, can rapidly catalyze and decompose straws, decompose the straws into nutritional ingredients for plants and beneficial organisms in a short time, reduce the use amount of pesticides and chemical fertilizers, develop natural ecological agriculture, and reduce environmental pollution.

The invention belongs to the field of agricultural fertilizers, and particularly relates to a multi-elementary fulvic acid fluid water-soluble fertilizer and a preparation method thereof. The fertilizer is easy to be absorbed by crops, fast in absorbing speed, and high in efficiency. The multi-elementary fulvic acid fluid water-soluble fertilizer is prepared from, by weight, not less than 260-508g/L of massive elements nitrogen+ phosphorus+ potassium, not less than 10-15 g/L of trace elements iron, zinc, copper and boron, not less than 2g/L of cobalt, and not less than 60-90g/L of humic acid. The multi-elementary fulvic acid fluid water-soluble fertilizer is prepared by a certain preparation method.

The invention discloses a traditional Chinese medicine foliar fertilizer, prepared from the following traditional Chinese medicine raw materials in weight: 5000 grams of astragalus root, 8000 grams of bighead atractylodes rhizome, 6000 grams of eucommia, 6000 grams of morinda root, 7000 grams of lance asiabell root, 200 grams of borneol, 4000 grams of amargosa bark, 5000 grams of baikal skullcap root, 5500 grams of dried orange peel, 4500 grams of Chinese atractylodes and 3000 grams of chuanxiong rhizome. The traditional Chinese medicine foliar fertilizer has the advantages of no pollution, lodging resistance, freezing resistance, disease and pest resistance, low cost and good effect, and can improve the yield and product quality of crops.

The invention discloses a biological agent capable of improving air environment and promoting plant photosynthesis and a preparation method thereof, and belongs to the technical field of organic agriculture. The invention aims to provide the biological gent capable of improving air environment and promoting plant photosynthesis, the yield and quality of crops can be improved, the investment cost is reduced, the soil structure is improved, the living environment of people is improved, and the fertilizer damage and medicine damage are reduced. The biological agent disclosed by the invention comprises the following components in parts by mass: 3 to 20 parts of amino acids or humic acid, 0.5 to 5 parts of plant antioxidant enzyme, 0.1 to 10 parts of phytagglutinin, 0.5 to 5 parts of oligosaccharides, 1 to 10 parts of a chelating agent and the balance of water. The biological agent disclosed by the invention is used for soil activation improvement and plant photosynthesis strengthening in agriculture, and has the effects of resisting diseases, expelling parasite, storing water and maintaining fertilizer.

The invention belongs to the field of agricultural fertilizer, and in particular relates to liquid fertilizer containing amino acids, zinc and selenium, and a preparation method of the liquid fertilizer. The liquid fertilizer is easily absorbed by crops, the quality of agricultural products is greatly improved, and conditions for producing functional foods are achieved. The liquid fertilizer containing the amino acids, the zinc and the selenium is prepared from the following components: more than or equal to 120-150g/L of the amino acids, more than or equal to 12g/L of zinc element and more than or equal to 80-100g/L of the selenium; the liquid fertilizer is prepared by the certain preparation method.

The invention belongs to the field of multifunctional novel bio-fertilizers and relates to a special plant source liquid medicinal fertilizer for melons and fruits. The fertilizer is prepared from the following raw materials in parts by weight: 2-9 parts of honeysuckles, 2-10 parts of mint, 1-7 parts of pseudo-ginseng, 7-20 parts of fulvic acid, 2-9 parts of brown sugar, 1-9 parts of amino-oligosaccharin, 2-9 parts of zinc sulfate, 2-7 parts of monopotassium phosphate, 20-70 parts of urea, 3-9 parts of boric acid, 0.01-0.1 part of lecithin and a proper amount of water. The plant source liquid medicinal fertilizer for melons and fruits prepared by the method provided by the invention is free from toxicity, pollution and public nuisance and is especially suitable for developing green agriculture, so that produced melons and fruits and the like meet the requirements of green food. The fertilizer has the characteristics of no residues, no pollution and no resistance agriculturally. The purposes of improving the quality of melons and fruits and enriching output and harvest are realized by virtue of adjusting endogenous hormones of melons and fruits.