The invention discloses a power supply device of a semiconductor laser and a constant-current source. The power supply device at least comprises an acquisition card, a first power supply, a second power supply and a third power supply, wherein the acquisition card is used for generating an input signal, the first power supply is connected with the acquisition card and the semiconductor laser, thesecond power supply is used for providing a working voltage for the first power supply, the third power supply is used for providing a working voltage for the semiconductor laser, the first power supply comprises an operational amplifier, a negative feedback circuit, a transistor, a diode and a first resistor, a first input end of the operational amplifier is connected with the acquisition card, asecond input end of the operational amplifier is connected with an output end of the operational amplifier, a first end of the transistor is connected with an output end of the operational amplifier,a second end of the transistor is connected with the semiconductor laser, and a third end of the transistor is connected with ground by the first resistor. By the mode, large-power, rapid-response and stable current input can be achieved, and the test efficiency of a semiconductor laser chip is improved.