This invention discloses a multi-channel light-slice
microscopic imaging device. The
image acquisition module includes
multiple image detectors. A
laser array emits multiple excitation beams of different wavelengths, which are then combined into a single
incident beam by an
optical path module. This
incident beam passes through a scanning
galvanometer and an imaging module before illuminating the sample on a stage mounted on a three-dimensional displacement platform. The imaging objective in the imaging module collects
fluorescence signals from the sample, which are then split by the beam-splitting module to form multiple
fluorescence signals of different wavelengths and projected onto the
multiple image detectors. Each
image detector forms an image imaging channel.
Multiple image imaging channels receive multiple
fluorescence signals of different wavelengths and transmit them to the corresponding image detectors. The three-dimensional displacement platform enables three-dimensional displacement movement of the sample slice. By employing a strategy of continuous motion imaging of the sample slice and simultaneous detection and acquisition of fluorescence signals by
multiple image detectors, the time interval between
sample preparation and imaging, as well as the pauses during imaging, can be reduced.