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1results about How to "Reduce displacement error" patented technology

A multi-channel light-sheet microscopic imaging device and its operation method

ActiveCN116360087BShorten the time intervalReduced imaging pausesMicroscopesMicro imagingLaser array
This invention discloses a multi-channel light-slice microscopic imaging device. The image acquisition module includes multiple image detectors. A laser array emits multiple excitation beams of different wavelengths, which are then combined into a single incident beam by an optical path module. This incident beam passes through a scanning galvanometer and an imaging module before illuminating the sample on a stage mounted on a three-dimensional displacement platform. The imaging objective in the imaging module collects fluorescence signals from the sample, which are then split by the beam-splitting module to form multiple fluorescence signals of different wavelengths and projected onto the multiple image detectors. Each image detector forms an image imaging channel. Multiple image imaging channels receive multiple fluorescence signals of different wavelengths and transmit them to the corresponding image detectors. The three-dimensional displacement platform enables three-dimensional displacement movement of the sample slice. By employing a strategy of continuous motion imaging of the sample slice and simultaneous detection and acquisition of fluorescence signals by multiple image detectors, the time interval between sample preparation and imaging, as well as the pauses during imaging, can be reduced.
Owner:INST OF ADVANCED TECH UNIV OF SCI & TECH OF CHINA +1

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