The invention provides a simulation test system for an industrial refrigerating unit, and a testing method applied to the system. The system comprises a simulation system for the industrial refrigerating unit, and a main control system for the industrial refrigerating unit, wherein the main control system is connected with the simulation system. The simulation system comprises a PLC, a WinMoD simulation unit, and an SIMBApro simulation unit, wherein the WinMoD simulation unit and the SIMBApro simulation unit are connected with the PLC. The main control system comprises the PLC and a man-machine interface. The WinMoD simulation unit is used for simulating I/O channels of all devices in the industrial refrigerating unit, and the SIMBApro simulation unit is used for simulating the I/O channels and the function of terminal equipment through a bus DP/PA. The system provided by the invention can completely simulate the condition of on-site equipment and an on-site bus, can achieve the simulation tests of all devices, avoids variable errors or dot mark leakage during on-site debugging, saves a large amount of time for on-site debugging, achieves the real seamless joint of a PLC program with simulation software, and employs a PC to debug the PLC program. When the system is used for debugging the PLC program, the testing efficiency is high, and the on-site debugging time is saved. Moreover, the energy is saved, and the stability of a program is good.