The invention discloses a preparation process of a negative oxygen ion coating, wherein the preparation process comprises the following steps: sequentially and slowly putting a filler and graphene into a sample machine according to a ratio, grinding the filler and the graphene, putting the mixture into a constant-temperature reaction device again after grinding is completed, mixing and stirring in the adding process, raising the temperature to 45-65 DEG C after adding is completed, and continuously mixing and stirring; and after completion, adding a tackifier, an anti-freezing agent, a dispersing agent, a film-forming agent, a defoaming agent and an antioxidant into a stirrer again, mixing and stirring again, continuing to add a pigment into the stirrer, and raising the temperature to 80-100 DEG C again. The hydraulic pressure supplied into elastic circulating pipes is increased, the elastic circulating pipes are expanded and increased, and the adjacent elastic circulating pipes extrude each other, so that the internal volume of the reaction kettle is reduced, the liquid level of a material is quickly raised, and a booster pump is closed after a proper height is reached, so that the material can be in full contact with the elastic circulating pipes at the moment.