44 results about "Hybrid vector" patented technology

Dynamic warp map generation system and corresponding method are disclosed. A compactor obtains spatial transformation parameters along with geometric and optical distortion parameters and combines them to form hybrid grid data in a hybrid vector space. This vector space is divided into hybrid blocks. In each hybrid block, the grid dataset is fitted with a hypersurface and the surface coefficients are saved in an interface. A decompactor obtains dynamic control parameters representing varying distortion parameters and generates a hybrid space vector. According to the hybrid space vector, a warp map is decoded from the hypersurface coefficients that compensates for dynamic geometric and optical distortions. In another example of the present invention, the dynamic warp map generation system is used for color gamut transformation.

The present invention provides for a hybrid vector system for the purpose of therapeutic gene delivery where the system is used for a targeted integration of a therapeutic gene into a genome. The hybrid vector system comprises a hybrid vector made up of a non-integrating lentiviral vector and an adeno-associated vector, and a therapeutic gene.

Vector halftoning and error diffusion are combined to provide a quantization method that yields high quality rendered images while demanding fewer system resources. For instance, the method is tolerant of resolution reductions in secondary or auxiliary channels to the vector halftoning process. Accordingly, these secondary pixel data channels can be sub-sampled and / or bit-depth reduced for transmission bandwidth conservation and / or reduction in data storage requirements. Restoring the resolution of the low resolution channels provides estimated image data to arrange or align with high resolution target channel data for the vector halftoning process. Error from marking decisions generated by the vector halftoning process is diffused to neighboring unprocessed pixels. The method also allows for the use of a small vector halftone threshold array while providing quantized images with fine texture and wide color gamuts. In some embodiments error diffusion is distributed according to vector error diffusion.

A method of reducing peak-to-average power in a hybrid signal is provided. The method determines peaks in power by defining a sample point by way of a digital vector and an analog vector. The digital and analog vectors are added together to generate a hybrid vector which is used to compare the sample point to the maximum desired peak threshold. An error vector is used to correct the sample point to a desired power level. Once the sample point has been corrected it can be added back to the analog signal and transmitted.

The beet novel cytoplasmic male sterile (P-CMS) line is a male sterile line containing wild beet cytoplasm, which is obtained by separating and screening in a hybridized descendant with a core substitution and separation selection method by hybridizing wild beet seed (B.patula Ait.) with sugar beets by the Heilongjiang University. The male sterile line is cultured into a single-seed cytoplasm male sterile line with the wild beet seed B.patula Ait. cytoplasm by multiple improvements. The scientific research result can adopt the cytoplasm male sterile line with the proprietary intellectual property rights as a hybridized carrier for genetic improvement and for widening the genetic basis of the Chinese beet so as to culture and combine to obtain the new beet varieties with the excellent genetic resource. According to the implementation of the invention item, the Chinese beet seed culturing work is like a winding path along mountain ridges on the genetic basis, is not limited by others, and is improved on the genetic diversity to a large extent.

The invention discloses a method for detecting interaction of proteins and relates to a detection method of proteins. The detection method comprises the following steps of: respectively cloning two interacting proteins to be detected on a bacterial two-hybrid vector and co-transforming to report bacteria; starting transcriptional expression of the report genes because of the interaction of the two target proteins, and generating special enzymes; dyeing the report bacteria co-transformed with the two target proteins by using a fluorogenic substrate, hydrolyzing the fluorogenic substrate by using the report product, that is, enzyme, to release a fluorescein, and detecting the interaction of the two target proteins in a single-bacterium level by quantitatively analyzing fluorescence intensity of the single bacterium by using a flow cytometer. The quantitative detection and correlation analysis of the protein-protein interaction in the single-bacterium level are important for accurate analysis of protein functions, certainty of signal transduction paths and discovery of new drug targets.

The invention discloses a rapid test paper strip detection method for directly amplifying seven hot spot mutation sites (R111X, IVS4-1, Y204C, R243Q, W326X, Y356X and R413P) of a human PAH gene without extraction, and a kit. The rapid test paper strip detection method comprises: directly adding a treatment liquid to a collected sample to prepare a sample liquid; carrying out identification and amplification on SNP allele sites based on an AS-PCR method; and achieving rapid genotyping by using the interaction between amplification fragment labeled digoxin and digoxin monoclonal antibody on thesurface of nanogold magnetic microparticles and by combining with a lateral flow chromatography technology. According to the present invention, with the rapid test paper strip detection method, the DNA separation extraction process is not performed, and the surface modified magnetic gold microparticles are used as the hybrid carrier, such that the problems of high cost, time saving and labor saving due to nucleic acid purification and product treatment can be eliminated, and high sensitivity and high accuracy are achieved.

Dynamic warp map generation system and corresponding method are disclosed. A compactor obtains spatial transformation parameters along with geometric and optical distortion parameters and combines them to form hybrid grid data in a hybrid vector space. This vector space is divided into hybrid blocks. In each hybrid block, the grid dataset is fitted with a hypersurface and the surface coefficients are saved in an interface. A decompactor obtains dynamic control parameters representing varying distortion parameters and generates a hybrid space vector. According to the hybrid space vector, a warp map is decoded from the hypersurface coefficients that compensates for dynamic geometric and optical distortions. In another example of the present invention, the dynamic warp map generation system is used for color gamut transformation.

The invention discloses a binary network realization system for recognition of common speech words. Common speech words are recognized using a binary convolution network. The circuit structure includes an Exclusive OR multiplier, a digital-analog hybrid vector matrix summing module, and a counting quantification based on a mixed clock frequency. The system is applied to keyword speech recognition,convolution neural network binaryzation and approximate adder design. The system not only can reduce the power consumption and time produced by calculation, but also can ensure the calculation precision to a certain degree and simplify the complexity of calculation.

Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3′ termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.

The beet novel cytoplasmic male sterile (M-CMS) line is a male sterile line containing wild beet cytoplasm, which is obtained by separating and screening in a hybridized descendant with a core substitution and separation selection method by hybridizing wild beet seed B.maritima L. with sugar beets by the Heilongjiang University. The male sterile line is cultured into a single-seed cytoplasm male sterile line (M-CMS) with the wild beet B.maritima L. cytoplasm by multiple improvements. The scientific research result can adopt the cytoplasm male sterile line with the proprietary intellectual property rights as a hybridized carrier for genetic improvement and for widening the genetic basis of the Chinese beet so as to culture and combine to obtain the new beet varieties with the excellent genetic resource. According to the implementation of the invention item, the Chinese beet seed culturing work is not limited by others on the genetic basis, and is improved on the genetic diversity to a large extent.

The invention discloses a multi-view remote sensing image fusion method, relates to the technical field of geographic information, and aims to solve the problem of low registration precision caused by limitation of registration of non-rigid transformation of an existing registration technology to ground surface fluctuation change or image multi-view change. The method comprises the steps of step 1, obtaining multiple groups of remote sensing images of different view angles in a same position through a satellite map or an unmanned aerial vehicle; step 2, extracting feature points of the multiple groups of the remote sensing images obtained in the step 1 by adopting a scale invariant feature transform algorithm to obtain a dot matrix (a) and a dot matrix (b); and step 3, registering the dot matrixes (a and b) in different states with a non-rigid dot matrix registration method based on global and local hybrid vectors and features, transforming a corresponding relationship of the remote sensing images obtained in the step 3 before and after view transformation by utilizing affine transformation, and outputting a registration result, so as to realize the fusion of the multi-view remote sensing images. The method is used for image fusion of the images during multi-view transformation.

The present invention relates to a genic expression adenoviral hybrid vector characterized in that it contains at least the following elements, oriented in the direction 5′ to 3′: i. a first chain of adenoviral origin comprising a first inverted terminal repeat (ITR) sequence and a signal sequence for packaging of the adenovirus; ii. a first non-encoding stuffer sequence; iii. a sequence corresponding to a tissue specific promoter; iv. a chain of cDNA derived from an alphavirus, the sequence of which is partly complementary to an alphaviral RNA sequence, comprising at least a sequence encoding for at least one exogenous gene of interest; v. a polyadenylation sequence; and vi. a second adenoviral inverted terminal repeat (ITR) sequence, it preferably relates to an adenoviral hybrid vector comprising as exogenous gene of interest the therapeutic gene of mammalian interleukin IL-12 and even more preferably human interleukin hIL-12; and to the use of the hybrid vector in a process for transferring genetic material to a cell, particularly a tumor cell that preferably expresses alpha-fetoprotein (AFP), and to its use for inducing an immune response against foreign antigens.

The invention relates to a method for using alginic acid-calcium carbonate hybrid gel to immobilize beta-glucuronidase, wherein, calcium carbonate powdered precipitation is generated by mixing and stirring sodium carbonate solution and calcium chloride solution at room temperature, and then kept, washed by water and acetones and dried, and micron mesoporous calcium carbonate particles are obtained which are added into trihydroxymethyl aminomethane-hydrochloric acid (Tirs-HCl) buffer of beta-glucuronidase or aqueous solution thereof for absorption, and calcium carbonate particles absorbing glucuronidase are obtained after centrifugal separation, then uniformly mixed with sodium alginate solution and added into calcium chloride solution by dropping for aging. The invention has the advantages of mild preparation conditions, simple and easy preparation technologies, good immobilization ability of obtained hybrid vectors on enzymes, low leakage rate of immobilized beta-glucuronidase, low swelling capacity and good reusage stability.

The invention discloses a method for detecting single nucleotide polymorphism (SNP) based on linker polymerase chain reaction (Linker-PCR) and magnetic nano particles. The method is characterized in that: whole genome amplification of a sample is carried out by means of Linker-PCR; a biotin-labeled amplification target sequence is fixed on avidin-labeled magnetic nano particles; and sample typing is realized through adopting a method for hybridizing with an allele specific probe. The method makes use of Linker-PCR to realize the synchronous detection and typing of multiple sites; moreover, through taking the magnetic nano particles as hybrid vectors, the method can avoid the disadvantages of high cost, time consumption and labor waste caused by target sequence purification and concentration; therefore, the method has the advantages of multiple sites, low cost, quickness and simplicity and convenience.

A hybrid adenovirus Semliki Forest Virus (SFV) vector includes 3′ and 5′ inverted terminal repeat (ITR) of adenovirus, the packaging signal of adenovirus, the structural genes encoding the adenovirus hexon and penton proteins, fiber and knob proteins and that may be deleted in the E4 region, E2 region or in the both the E2 and E4 regions. The adenovirus vector may not require a helper virus coinfection for propagation in producer cell lines. A hybrid vector includes a eukaryotic promoter controlling expression of the 42S genome of SFV comprising the nonstructural genes 1-4 or two point mutations thereof, and the therapeutic mRNA, in the cytoplasm. In use, the hybrid vector further comprises cDNA encoding for microRNA (miRNA) and hairpin loops of short interfering RNA (siRNA) or cDNA encoding for double-stranded RNA (dsRNA).

The invention discloses a method for improving conjugational transfer efficiency of fluorescent plasmid transfer of fluorescence labeling strains of rhizobia by taking sterilizing alfalfa leaves as triparental mating carriers instead of sterile filter membranes. The method can be used for efficiently constructing CFP (cyan fluorescent protein) gene labeled strains of rhizobia. The method specifically comprises the following steps of taking small and tender leaves of alfalfa, uniformly putting the leaves in a single layer in a culture dish after washing the leaves with distilled water, and sterilizing the leaves; taking out the leaves with a pair of sterile tweezers, sticking the leaves to the surface of TY culture media, and covering the culture dish; and using the leaves to replace microporous filter membranes in the triparental mating process to carry out mixed growing culture on donors, assistant bacteria and recipient bacteria. Compared with the microporous filter membranes as the mating carriers, the alfalfa leaves, as the mating carriers, can improve the conjugational transfer efficiency of the fluorescent plasmids by 8.4-11 times, and the difference is obvious. As the used raw materials are alfalfa plant leaves which are renewable and accessible, the method has the advantage that the raw materials are cheap and accessible, is simple and convenient to operate and is suitable for constructing the fluorescence labeling strains of rhizobia.

An analogue peptide that comprises the variable regions of the light or heavy chains of an antibody of a first species selectively binding to a carcinoma antigen has 1 to 46 amino acids of the framework regions per chain substituted with amino acids such as those present in equivalent positions in antibodies of a species other than the first species, or fragments thereof comprising 1 to 3 variable region CDRs per chain and optionally flanking regions thereof of 1 to 10 or more amino acids, alone or with an N-terminal fragment of 1 to 10 or more amino acids, combinations or mixtures thereof. The polypeptide may also comprise an effector agent and / or be glycosylated, and is presented as a composition with a carrier. The analogue peptides are used in diagnostic kits for carcinomas and methods for in vivo imaging and treating a primary or metastasized carcinoma, and in vitro diagnosing a carcinoma, ex vivo purging neoplastic cells from a biological fluid. RNAs and DNAs encode the analogue peptide, and a hybrid vector carrying the nucleotides and transfected cells express the peptides and a method produces the analogue peptide. An anti-idiotype polypeptide comprises polyclonal antibodies raised against an anti-carcinoma antibody or the analogue peptide of this invention, monoclonal antibodies thereof, Fab, Fab′, (Fab′)2, CDR, variable region, or analogues or fragments thereof, combinations thereof with an oligopeptide comprising a TRP trimer, tandem repeats thereof, or combination or mixtures thereof. An anti-idiotype hybrid polypeptide with an effector agent and the anti-idiotype polypeptide, an anti-carcinoma vaccine, an anti-carcinoma vaccination kit, a method of vaccinating against carcinoma and a method of lowering the serum concentration of a circulating antibody or polypeptide are provided.

The invention relates to purified proteins induced in human cells by interferon alpha or beta, RNAs, DNAs and hybrid vectors coding for said proteins, hosts transformed with such a hybrid vector, processes for the preparation and purification of these proteins, DNAs, vectors and hosts, monoclonal antibodies specific to these proteins, monoclonal antibody derivatives, hybridoma cell lines secreting these monoclonal antibodies, the use of the monoclonal antibodies and their derivatives in the qualitative and quantitative determination of these proteins, test kits containing the monoclonal antibodies, and pharmaceutical preparations containing said proteins. A protein of the invention shows antiviral properties ascribed to interferons and may be a valuable indicator of the cell response to an interferon therapy.

An expression vector capable of expressing high levels of heterologous proteins having a cytomegalovirus (CMV) enhancer 5′ upstream from a myeloproliferative sarcoma virus (MPSV) promoter.

It has been established that bacterial hybrid vectors including prokaryote cells modified by the addition of cationic polymers to the outer surface of the cell can selectively deliver exogenous cargos, such as nucleic acids, polypeptides and small molecules to an eukaryotic cell, such as an antigen presenting cell. Compositions and methods for the delivery and expression of nucleic acids and polypeptides to eukaryotic cells are described. The bacterial hybrid vectors include one or more cationic polymers that enhance uptake by antigen presenting cells. The hybrid bacterial vectors include expression vectors that express one or more factors to enhance lysosomal escape and cytosolic delivery of cargo. The vectors are useful as adjuvants to stimulate and / or induce immune responses to any desired antigen, to develop a protective immune response in a subject.

A method of reducing peak-to-average power in a hybrid signal is provided. The method determines peaks in power by defining a sample point by way of a digital vector and an analog vector. The digital and analog vectors are added together to generate a hybrid vector which is used to compare the sample point to the maximum desired peak threshold. An error vector is used to correct the sample point to a desired power level. Once the sample point has been corrected it can be added back to the analog signal and transmitted.

A method, system, apparatus, and article of manufacture provide the ability to conduct a hybrid raster / vector based paint operation. A user commences a raster based paint operation. Based on the raster operation, vector-based information is determined and recorded. The system then determines if a requested user operation requires the vector-based information. If the requested user operation requires the vector-based information, a representative raster based stroke is generated / recreated based on the vector-based information and the requested user operation is performed on the representative raster based stroke.

The invention belongs to the technical field of biology, and particularly relates to a pGADT7-In vector applicable to In-Fusion cloning as well as construction and use methods of the pGADT7-In vector.A multiple cloning site of pGADT7 is transformed with a site-directed mutagenesis technology, the pGADT7-In vector is obtained, after pGBKT7 vector and the pGADT7-In vector are subjected to enzyme digestion with EcoRI, formed tail ends contain the same sequence with length being 15 bp or longer, and further, vector construction is performed with the In-Fusion technology; only one pair of primersis required for construction of a bait vector or a prey vector of a given gene, and one-time PCR is performed; an obtained PCR product is subjected to an In-Fusion reaction with pGBKT7 or pGADT7, escherichia coli is transformed, LB flat plates with different resistance are coated with an obtained product, identification is performed by bacterial colony or bacterial liquid PCR, and construction ofthe bait vector or the prey vector is completed easily, rapidly and efficiently. With application of the pGADT7-In vector and the vector construction method to construction of a yeast two-hybrid vector, experimental cycle is greatly shortened, experimental cost is reduced, and success rate is increased.

The invention relates to the technical field of biology, in particular to a yeast two-hybrid vector, a construction method and application thereof in protein interaction. The yeast two-hybrid vector comprises a pNC-GADT7 vector and a pNC-GBKT7 vector. According to the pNC-GADT7 vector, a Nimble Cloning frame is inserted into a cloning site of the pGADT7 vector; and according to the pNC-GBKT7 vector, a Nimble Cloning frame is inserted into a cloning site of the pGBKT7 vector. The yeast two-hybrid vector of the Nimble Cloning is convenient for cloning a target gene to the vector, and has the advantages of simple operation, high cloning efficiency, standardized cloning and the like.

An analogue peptide that comprises the variable regions of the light or heavy chains of an antibody of a first species selectively binding to a carcinoma antigen has 1 to 46 amino acids of the framework regions per chain substituted with amino acids such as those present in equivalent positions in antibodies of a species other than the first species, or fragments thereof comprising 1 to 3 variable region CDRs per chain and optionally flanking regions thereof of 1 to 10 or more amino acids, alone or with an N-terminal fragment of 1 to 10 or more amino acids, combinations or mixtures thereof. The polypeptide may also comprise an effector agent and / or be glycosylated, and is presented as a composition with a carrier. The analogue peptides are used in diagnostic kits for carcinomas and methods for in vivo imaging and treating a primary or metastasized carcinoma, and in vitro diagnosing a carcinoma, ex vivo purging neoplastic cells from a biological fluid. RNAs and DNAs encode the analogue peptide, and a hybrid vector carrying the nucleotides and transfected cells express the peptides and a method produces the analogue peptide. An anti-idiotype polypeptide comprises polyclonal antibodies raised against an anti-carcinoma antibody or the analogue peptide of this invention, monoclonal antibodies thereof, Fab, Fab′, (Fab′)2, CDR, variable region, or analogues or fragments thereof, combinations thereof with an oligopeptide comprising a TRP trimer, tandem repeats thereof, or combination or mixtures thereof. An anti-idiotype hybrid polypeptide with an effector agent and the anti-idiotype polypeptide, an anti-carcinoma vaccine, an anti-carcinoma vaccination kit, a method of vaccinating against carcinoma and a method of lowering the serum concentration of a circulating antibody or polypeptide are provided.

The present invention relates to the field of genetic engineering technique, which is object to construct a bacterial single-hybrid vector, named pBXcmT, whose nucleotide sequence is shown as the sequence table SEQ ID NO: 1 and whose molecular weight is 3794563Da. The vector of the invention has highlight advantages that: it is fully compatible with the prior bacilli double-hybrid commercialization gene library, the report strain and other test reagents, in particular, it incorporates TA cloning, and can be easily and quickly used in the rapid cloning of short regulated nucleotide sequence. The bacterial single-hybrid vector has a wide range of applications in the identification and screening of DNA-protein interactions.

The invention relates to a method for immobilizing beta-glucuronidase by alginic acid-calcium carbonate hybrid gel. Sodium carbonate solution and calcium chloride solution are mixed and stirred at room temperature to generate calcium carbonate powder precipitation, which is left to stand, washed with water and acetone, and dried to obtain micron-sized mesoporous calcium carbonate particles. Adsorb in aminomethane-hydrochloric acid (Tirs-HCl) buffer solution or aqueous solution, and centrifuge to obtain calcium carbonate particles adsorbing glucuronidase and mix them evenly with sodium alginate solution, then add dropwise to calcium chloride solution, Ageing. The preparation condition of the invention is mild, the preparation process is simple and easy, the obtained hybrid carrier has good immobilization ability for enzyme, the leakage rate of the immobilized β-glucuronidase is low, the swelling degree is low, and the repeated use stability is good.

The invention discloses a related recombinant vector used for yeast two-hybrid and a yeast two-hybrid method for large-scale screening interacting protein using a high-flux sequencing technology. The method depends on a peculiar integrative function of an integrase Integrase phiC31(En), and by conducting transformation on a traditional yeast two-hybrid vector, the vector is capable of conducting organic combination with the high-flux sequencing technology, and thus that hundreds of bait protein can simultaneously conduct screening work on Prey protein library can be realized. The method comprises the steps of inserting the integrase and a specially recognized nucleotide sequence into a vector of a Matchmaker GAL4 system, making the recombinant vector enter into the same yeast cell through the yeast two-hybrid method, using the integrase to play a role in conducting specific orientational rearrangement on the specially recognized nucleotide sequence and a nucleotide sequence closely adjacent to the specially recognized nucleotide sequence so as to make two recombinant vectors combine into a combined vector, at this time the Prey protein and the bait protein are orientationally connected into one new section of sequence on the combined vector. Through amplification and high high-flux sequencing, the recombinant vector can conduct economic and efficient screening on interacting proteins and construct a comprehensive network atlas for interactions between the proteins.

The present invention relates to the field of genetic engineering technique, which is object to construct a bacterial single-hybrid vector, named pBXcmT, whose nucleotide sequence is shown as the sequence table SEQ ID NO: 1 and whose molecular weight is 3794563Da. The vector of the invention has highlight advantages that: it is fully compatible with the prior bacilli double-hybrid commercialization gene library, the report strain and other test reagents, in particular, it incorporates TA cloning, and can be easily and quickly used in the rapid cloning of short regulated nucleotide sequence. The bacterial single-hybrid vector has a wide range of applications in the identification and screening of DNA-protein interactions.