43 results about "Hybrid vector" patented technology

Dynamic warp map generation system and corresponding method are disclosed. A compactor obtains spatial transformation parameters along with geometric and optical distortion parameters and combines them to form hybrid grid data in a hybrid vector space. This vector space is divided into hybrid blocks. In each hybrid block, the grid dataset is fitted with a hypersurface and the surface coefficients are saved in an interface. A decompactor obtains dynamic control parameters representing varying distortion parameters and generates a hybrid space vector. According to the hybrid space vector, a warp map is decoded from the hypersurface coefficients that compensates for dynamic geometric and optical distortions. In another example of the present invention, the dynamic warp map generation system is used for color gamut transformation.

The present invention provides for a hybrid vector system for the purpose of therapeutic gene delivery where the system is used for a targeted integration of a therapeutic gene into a genome. The hybrid vector system comprises a hybrid vector made up of a non-integrating lentiviral vector and an adeno-associated vector, and a therapeutic gene.

A method of reducing peak-to-average power in a hybrid signal is provided. The method determines peaks in power by defining a sample point by way of a digital vector and an analog vector. The digital and analog vectors are added together to generate a hybrid vector which is used to compare the sample point to the maximum desired peak threshold. An error vector is used to correct the sample point to a desired power level. Once the sample point has been corrected it can be added back to the analog signal and transmitted.

The invention discloses a rapid test paper strip detection method for directly amplifying seven hot spot mutation sites (R111X, IVS4-1, Y204C, R243Q, W326X, Y356X and R413P) of a human PAH gene without extraction, and a kit. The rapid test paper strip detection method comprises: directly adding a treatment liquid to a collected sample to prepare a sample liquid; carrying out identification and amplification on SNP allele sites based on an AS-PCR method; and achieving rapid genotyping by using the interaction between amplification fragment labeled digoxin and digoxin monoclonal antibody on thesurface of nanogold magnetic microparticles and by combining with a lateral flow chromatography technology. According to the present invention, with the rapid test paper strip detection method, the DNA separation extraction process is not performed, and the surface modified magnetic gold microparticles are used as the hybrid carrier, such that the problems of high cost, time saving and labor saving due to nucleic acid purification and product treatment can be eliminated, and high sensitivity and high accuracy are achieved.

Dynamic warp map generation system and corresponding method are disclosed. A compactor obtains spatial transformation parameters along with geometric and optical distortion parameters and combines them to form hybrid grid data in a hybrid vector space. This vector space is divided into hybrid blocks. In each hybrid block, the grid dataset is fitted with a hypersurface and the surface coefficients are saved in an interface. A decompactor obtains dynamic control parameters representing varying distortion parameters and generates a hybrid space vector. According to the hybrid space vector, a warp map is decoded from the hypersurface coefficients that compensates for dynamic geometric and optical distortions. In another example of the present invention, the dynamic warp map generation system is used for color gamut transformation.

The invention discloses a binary network realization system for recognition of common speech words. Common speech words are recognized using a binary convolution network. The circuit structure includes an Exclusive OR multiplier, a digital-analog hybrid vector matrix summing module, and a counting quantification based on a mixed clock frequency. The system is applied to keyword speech recognition,convolution neural network binaryzation and approximate adder design. The system not only can reduce the power consumption and time produced by calculation, but also can ensure the calculation precision to a certain degree and simplify the complexity of calculation.

The beet novel cytoplasmic male sterile (M-CMS) line is a male sterile line containing wild beet cytoplasm, which is obtained by separating and screening in a hybridized descendant with a core substitution and separation selection method by hybridizing wild beet seed B.maritima L. with sugar beets by the Heilongjiang University. The male sterile line is cultured into a single-seed cytoplasm male sterile line (M-CMS) with the wild beet B.maritima L. cytoplasm by multiple improvements. The scientific research result can adopt the cytoplasm male sterile line with the proprietary intellectual property rights as a hybridized carrier for genetic improvement and for widening the genetic basis of the Chinese beet so as to culture and combine to obtain the new beet varieties with the excellent genetic resource. According to the implementation of the invention item, the Chinese beet seed culturing work is not limited by others on the genetic basis, and is improved on the genetic diversity to a large extent.

An expression vector capable of expressing high levels of heterologous proteins having a cytomegalovirus (CMV) enhancer 5′ upstream from a myeloproliferative sarcoma virus (MPSV) promoter.

The invention relates to the technical field of biology, in particular to a yeast two-hybrid vector, a construction method and application thereof in protein interaction. The yeast two-hybrid vector comprises a pNC-GADT7 vector and a pNC-GBKT7 vector. According to the pNC-GADT7 vector, a Nimble Cloning frame is inserted into a cloning site of the pGADT7 vector; and according to the pNC-GBKT7 vector, a Nimble Cloning frame is inserted into a cloning site of the pGBKT7 vector. The yeast two-hybrid vector of the Nimble Cloning is convenient for cloning a target gene to the vector, and has the advantages of simple operation, high cloning efficiency, standardized cloning and the like.

An analogue peptide that comprises the variable regions of the light or heavy chains of an antibody of a first species selectively binding to a carcinoma antigen has 1 to 46 amino acids of the framework regions per chain substituted with amino acids such as those present in equivalent positions in antibodies of a species other than the first species, or fragments thereof comprising 1 to 3 variable region CDRs per chain and optionally flanking regions thereof of 1 to 10 or more amino acids, alone or with an N-terminal fragment of 1 to 10 or more amino acids, combinations or mixtures thereof. The polypeptide may also comprise an effector agent and / or be glycosylated, and is presented as a composition with a carrier. The analogue peptides are used in diagnostic kits for carcinomas and methods for in vivo imaging and treating a primary or metastasized carcinoma, and in vitro diagnosing a carcinoma, ex vivo purging neoplastic cells from a biological fluid. RNAs and DNAs encode the analogue peptide, and a hybrid vector carrying the nucleotides and transfected cells express the peptides and a method produces the analogue peptide. An anti-idiotype polypeptide comprises polyclonal antibodies raised against an anti-carcinoma antibody or the analogue peptide of this invention, monoclonal antibodies thereof, Fab, Fab′, (Fab′)2, CDR, variable region, or analogues or fragments thereof, combinations thereof with an oligopeptide comprising a TRP trimer, tandem repeats thereof, or combination or mixtures thereof. An anti-idiotype hybrid polypeptide with an effector agent and the anti-idiotype polypeptide, an anti-carcinoma vaccine, an anti-carcinoma vaccination kit, a method of vaccinating against carcinoma and a method of lowering the serum concentration of a circulating antibody or polypeptide are provided.

The present invention relates to the field of genetic engineering technique, which is object to construct a bacterial single-hybrid vector, named pBXcmT, whose nucleotide sequence is shown as the sequence table SEQ ID NO: 1 and whose molecular weight is 3794563Da. The vector of the invention has highlight advantages that: it is fully compatible with the prior bacilli double-hybrid commercialization gene library, the report strain and other test reagents, in particular, it incorporates TA cloning, and can be easily and quickly used in the rapid cloning of short regulated nucleotide sequence. The bacterial single-hybrid vector has a wide range of applications in the identification and screening of DNA-protein interactions.

The invention relates to a method for immobilizing beta-glucuronidase by alginic acid-calcium carbonate hybrid gel. Sodium carbonate solution and calcium chloride solution are mixed and stirred at room temperature to generate calcium carbonate powder precipitation, which is left to stand, washed with water and acetone, and dried to obtain micron-sized mesoporous calcium carbonate particles. Adsorb in aminomethane-hydrochloric acid (Tirs-HCl) buffer solution or aqueous solution, and centrifuge to obtain calcium carbonate particles adsorbing glucuronidase and mix them evenly with sodium alginate solution, then add dropwise to calcium chloride solution, Ageing. The preparation condition of the invention is mild, the preparation process is simple and easy, the obtained hybrid carrier has good immobilization ability for enzyme, the leakage rate of the immobilized β-glucuronidase is low, the swelling degree is low, and the repeated use stability is good.

The present invention relates to the field of genetic engineering technique, which is object to construct a bacterial single-hybrid vector, named pBXcmT, whose nucleotide sequence is shown as the sequence table SEQ ID NO: 1 and whose molecular weight is 3794563Da. The vector of the invention has highlight advantages that: it is fully compatible with the prior bacilli double-hybrid commercialization gene library, the report strain and other test reagents, in particular, it incorporates TA cloning, and can be easily and quickly used in the rapid cloning of short regulated nucleotide sequence. The bacterial single-hybrid vector has a wide range of applications in the identification and screening of DNA-protein interactions.