The invention provides hot-melt three-proofing paint as well as a preparation method and a use method thereof. The three-proofing paint is prepared from 1-3% of toner, 1-3% of antioxidant, 3-5% of flexibilizer, 20-30% of tackifier and 60-70% of polyolefin. The antioxidant is beta-(3,5-di-tert-butyl-4-hydroxyphenyl)propionic acid isooctyl alcohol ester, the tackifier is one or more of C5 petroleumresin, poly alpha methyl styrene resin and hydrogenated terpene resin, and the polyolefin is one or more of polyethylene, polypropylene, poly-1-butene and poly-4-methyl-1-pentene. No solvent is addedinto the raw materials of the hot-melt three-proofing paint, and the hot-melt three-proofing paint is in a solid state during storage and transportation, so that potential safety hazards caused by solvent volatilization and leakage in the storage process are avoided, the problems of package swelling and damage caused by solvent volatilization due to temperature change in the storage process are also avoided, and the storage stability is high. In the using process, no solvent is volatilized, heating or ultraviolet irradiation auxiliary curing is not needed, and using is convenient, safe and environmentally friendly.