The invention relates to a bio-oil starch adhesive for wood, which belongs to the technical field of adhesive preparation. A preparation method comprises the following steps: carrying out acidolysis, oxidation and denaturation on starches by using a hydrochloric acid and an oxidant so as to reduce the molecular weight of the starches and improve the reactivity of the starches; then, adding an emulsifier and a stabilizer into the obtained object, and carrying out graft copolymerization on the obtained mixture and acrylamide under the action of an initiator; adding bio-oil, so that chemical groups on a skeleton structure are modified by using substances such as acid and aldehyde and the like in the bio-oil, thereby obtaining a host adhesive; and finally, adding a crosslinking agent and packing into the host adhesive so as to obtain the bio-oil starch adhesive, wherein the mass part of each raw material component is as follows: 80-120 parts of the starches, 150 parts of the hydrochloric acid, 2-5 parts of the oxidant, 1-3 parts of the emulsifier, 10-20 parts of the stabilizer, 0.3-0.6 part of the initiator, 10-20 parts of the acrylamide, 5-20 parts of the bio-oil, 5-10 parts of the crosslinking agent, and 10-15 parts of the packing. The bio-oil starch adhesive disclosed by the invention can replace a white emulsion and can be widely used in the aspects of wood ornaments, wood processing and artificial boards.